姜黄素通过降低压力超负荷心肌肥厚大鼠循环微颗粒水平改善内皮功能发挥心脏保护作用  

Curcumin improves endothelial function and plays a cardio-protective effect by reducing level of circulating microparticles in pressure overload-induced myocardial hypertrophy in rats

作　　者：崔欣欣 白晓洁[1] 王起越 李俊虎 曹茜倩 林媛媛[3] 封启龙[1] CUI Xin-xin;BAI Xiao-jie;WANG Qi-yue;LI Jun-hu;CAO Xi-qian;LIN Yuan-yuan;FENG Qi-long(Dept of Physiology, Key Laboratory of Cellular Physiology ,Ministry of Education, Key Laboratory of Cellular Physiology, Shanxi Province, Shanxi Medical University, Taiyuan 030001, China;Dept of Clinical Research of Taiyuan Health School, Taiyuan 030012, China;Dept of Cardiology, Third Hospital of Shanxi Medical University, Shanxi Bethune Hospital, Taiyuan 030032, China)

机构地区：[1]山西医科大学基础医学院生理学系,细胞生理学教育部重点实验室,细胞生理学山西省重点实验室,山西太原030001 [2]太原市卫生学校临床教研室,山西太原030012 [3]山西医科大学第三医院山西白求恩医院心血管内科,山西太原030032

出　　处：《中国药理学通报》2021年第7期916-921,共6页Chinese Pharmacological Bulletin

基　　金：山西省自然基金资助项目(No 201901D111195);山西省‘1331工程’重点学科建设计划经费;细胞生理学教育部重点实验室(山西医科大学)开放基金(No KLMEC/SXMU-202007)。

摘　　要：目的观察姜黄素对心肌肥厚大鼠的心脏保护作用,并探索其作用机制。方法AAC法建立SD大鼠压力超负荷心肌肥厚模型,实验分为Sham组、AAC组和姜黄素组。术后16周给予灌胃治疗;术后20周,超声心动图评估大鼠心脏功能;生物机能实验系统采集大鼠心率变化、血压变化和左室内压指标;BCA法检测各组大鼠MPs蛋白浓度;CCK-8法检测各组MPs对HUVEC活力的影响。结果术后20周,仅AAC组死亡2只大鼠。与Sham组相比,AAC组结论姜黄素对压力超负荷心肌肥厚大鼠具有明显的心脏保护效应,推测其机制与降低大鼠循环MPs水平,提高内皮细胞存活率有关。Aim To observe the cardio-protective effects of curcumin on rats with myocardial hypertrophy,and to further explore the mechanism.Methods Abdominal aorta was constricted in SD rats to establish a pressure overload-induced myocardial hypertrophy model.Rats were divided into sham group,AAC group and curcumin group.They were treated by intragastric administration.Twenty weeks after the operation,cardiac function was evaluated using echocardiogram.The heart rate was recorded using biological function experiment system.The blood pressure and left ventricular pressure were measured by a cannulation into right common carotid artery and left ventricular respectively.Circulating blood MPs level in rats was detected by BCA.Effects of MPs on the viability of human umbilical vein endothelial cell were measured by CCK-8.Results Twenty weeks after surgery,only two rats in AAC group died.The results of echocardiography showed that compared with sham group,left ventricular internal dimension at systole(LVIDs),left ventricular internal diastolic dimension(LVIDd),left ventricular posterior wall diameter(LVPWd),heart rate(HR),mean arterial pressure(MAP)and left ventricular end-diastolic pressure(LVEDP)all significantly increased in AAC group,whereas ejection fraction(EF),fractional shortening(FS),left ventricular systolic pressure(LVSP),maximum upstroke velocity of left ventricular pressure(+dp/dtmax)and maximum descent velocity of the first derivative of left ventricular pressure(-dp/dtmax)were significantly reduced.Curcumin dramatically improved the above abnormalities.The protein concentration of MPs from AAC group was significantly higher than that from sham group,and the protein content of MPs of curcumin group decreased compared with that of AAC group.MPs from AAC group significantly inhibited endothelial cell viability.However,cell viability was higher in curcumin group than in AAC group.Conclusions Curcumin has a significant cardio-protective effect on pressure overload-induced myocardial hypertrophy rats,which may be relat

关 键 词：心肌肥厚 姜黄素 微颗粒 内皮细胞 心功能 血流动力学 

分 类 号：R-332[医药卫生] R329.24