HRP催化共振散射光谱法测定食品接触材料中双酚A  

Determination of bisphenol A in food contact materials by HRP catalytic resonance scattering spectrometry

作　　者：梁泉 陈佩利 吴小东 LIANG Quan;CHEN Peili;WU Xiaodong(Zhejiang Academy of Ecological Environment Design and Research,Hangzhou 310007)

机构地区：[1]浙江省生态环境科学设计研究院,杭州310007

出　　处：《分析试验室》2021年第6期718-721,共4页Chinese Journal of Analysis Laboratory

摘　　要：基于在辣根过氧化物酶(HRP)催化作用下,过氧化氢(H2O2)可快速氧化双酚A(BPA)并促使BPA发生聚合,聚合产物进而与β-环糊精(β-CD)发生包合作用产生共振散射(RLS)增强信号,于328 nm波长处产生共振散射特征峰,峰高与BPA含量成正相关性。由此建立了食品接触材料中BPA的共振散射测定新方法。方法的线性范围为1.0×10^(-3)~0.50 mg/L,检出限为3×10^(-4)mg/L,样品加标回收率为94.0%~103.3%。方法适宜于基层实验室食品接触材料中BPA的检测。In the presence of horseradish peroxidase(HRP),hydrogen peroxide(H2O2)can rapidly oxidize bisphenol A(BPA)and promote BPA polymerization.The polymerization product can then be encapsulated withβ-cyclodextrin(β-CD)to generate resonance scattering(RLS)enhancement signal.The resonance scattering characteristic peak appears at 328 nm wavelength,and the peak height is positively correlated with BPA content.Based on it,a new resonance scattering method for the determination of BPA in food contact materials was established.The linear range of the method was 1.0×10^(-3)-0.50 mg/L,and the detection limit was 3×10^(-4) mg/L with the recoveries of 94.0%-103.3%.The method is suitable for the detection of BPA in food contact materials in primary laboratory.

关 键 词：催化氧化 共振散射光谱法 食品接触材料 双酚A H2O2 

分 类 号：O657.31[理学—分析化学]