卤醇脱卤酶研究进展  

Research progress on halohydrin dehalogenase

作　　者：丁俊辉 袁林 喻晨[2] 侯亚利 杨忠华 全灿[3] DING Junhui;YUAN Lin;YU Chen;HOU Yali;YANG Zhonghua;QUAN Can(School of Chemistry and Chemical Engineering,Wuhan University of Science and Technology,Wuhan 430081,China;Angel Yeast Co.,Ltd.,Yichang 443000,China;Division of Chemical Metrology and AnalyticalScience,National Institute of Metrology,Beijing 100013,China)

机构地区：[1]武汉科技大学化学与化工学院,湖北武汉430081 [2]安琪酵母股份有限公司,湖北宜昌443000 [3]中国计量科学研究院化学计量与分析科学研究所,北京100013

出　　处：《发酵科技通讯》2021年第2期119-124,共6页Bulletin of Fermentation Science and Technology

基　　金：国家重点研发计划资助项目(2017YFF0205803)。

摘　　要：卤醇脱卤酶是合成手性纯的环氧化物及β-取代醇的关键生物催化剂。通过分子内亲核取代机制催化邻卤醇脱卤生成手性的环氧化物,在亲核试剂介导的作用下催化环氧化物开环生成手性的β-取代醇,在手性医药中间体合成中具有重要应用。通过分子改造和固定化可以使卤醇脱卤酶的酶活、立体选择性和稳定性显著提高,对其工程应用具有重要意义。对近些年卤醇脱卤酶的来源、分子改造和固定化的研究进展进行综述,并对未来的研究方向进行初步探讨,以期为卤醇脱卤酶研究提供帮助。Halohydrin dehalogenase is the key biocatalyst to produce chiral epoxides and chiral andβ-substituted alcohol.Halohydrin dehalogenase can catalyze the dehalogenation of o-halo alcohol to produce chiral epoxides following the intramolecular nucleophilic substitution mechanism.It also can catalyze the opening ring reaction of halogenated epoxy compounds to synthesis chiralβ-substituted alcohol.The products,chiral epoxides andβ-substituted alcohol are key chiral building blocks for chiral pharmaceuticals and other chiral fine chemicals.The molecular evolution and immobilization of the halohydrin dehalogenase can significantly improve the enzyme activity,stereoselectivity and stability,which is very important to halohydrin dehalogenase application.In this review,the research progress on the halohydrin dehalogenase source,molecular evolution and immobilization in recent years is highlighted.The further research direction about halohydrin dehalogenase is also pointed out.

关 键 词：卤醇脱卤酶 卤化物 分子进化 酶固定化 

分 类 号：Q814[生物学—生物工程]