miR-126通过靶向调控Crk基因抑制非小细胞肺癌细胞增殖及侵袭  

miR-126 inhibits the proliferation and invasion of non-small cell lung cancer cells by targeting the Crk gene

作　　者：刘艳红 姚菲菲 王富霞 LIU Yanhong;YAO Feifei;WANG Fuxia(Department of Respiratory and Critical Care Medicine,People's Hospital of Henan University of Traditional Chinese Medicine,Zhengzhou People's Hospital,Henan Zhengzhou 450000,China.)

机构地区：[1]河南中医药大学人民医院/郑州人民医院呼吸与危重症医学科,河南郑州450000

出　　处：《现代肿瘤医学》2021年第13期2219-2223,共5页Journal of Modern Oncology

基　　金：河南省自然科学基金资助项目(编号:20182142301)。

摘　　要：目的:探究miR-126通过靶向调控Crk基因来调控非小细胞肺癌(NSCLC)细胞增殖及侵袭。方法:应用实时定量PCR检测NSCLC组织及其癌旁肺组织中miR-126和Crk mRNA的表达。应用荧光素酶报告基因系统验证Crk是miR-126的靶基因。在NSCLC细胞转染miR-126 mimics或特异沉默Crk的siRNA后,用MTT法检测NSCLC细胞增殖能力,Transwell测定NSCLC细胞侵袭能力。结果:与癌旁肺组织相比,miR-126在肺癌组织中表达显著降低(P<0.05),Crk mRNA在肺癌组织中表达明显升高(P<0.05)。miR-126直接作用于Crk-3'UTR的预计靶位点;MTT细胞增殖实验及Transwell侵袭实验表明,miR-126过表达或Crk基因沉默均能减弱肺癌细胞增殖及侵袭能力(P<0.05)。结论:miR-126通过靶向调控Crk来抑制NSCLC细胞的增殖及侵袭。Objective:To investigate how miR-126 regulates the proliferation and invasiveness of NSCLC cells by targeting the Crk gene.Methods:Real-time quantitative PCR was used to detect the expressions of miR-126 and Crk mRNA in NSCLC tissues and adjacent lung tissues.The luciferase reporter gene system was used to verify that Crk was the target gene of miR-126.After NSCLC cells were transfected with miR-126 mimics or specific silenced Crk siRNA,the proliferation of NSCLC cells was detected by MTT assay,and the invasiveness of NSCLC cells was determined by Transwell.Results:Compared with adjacent tissues,miR-126 expression was significantly reduced in cancer tissues(P<0.05),and Crk mRNA expression was significantly increased in lung cancer tissues(P<0.05).miR-126 directly acted on the predicted target sites of Crk-3'UTR.MTT cell proliferation test and Transwell invasion test showed that miR-126 overexpression or Crk gene silencing could reduce the proliferation and invasion ability of lung cancer cells(P<0.05).Conclusion:miR-126 inhibits the proliferation and invasion of NSCLC cells by targeting Crk.

关 键 词：MIR-126 CRK 非小细胞肺癌 增殖 侵袭 

分 类 号：R734.2[医药卫生—肿瘤]