酵母甘露聚糖(20 kDa)的生物安全性及体内分布研究  

The biosafety in vitro and the distribution in vivo of yeast mannan with 20 kDa molecular weight

作　　者：付乾振 王佳奇 樊坤 刘洁 王子朝 张慧茹[1] FU Qianzhen;WANG Jiaqi;FAN Kun;LIU Jie;WANG Zichao;ZHANG Huiru(College of Biological Engineering,Henan University of Technology,Zhengzhou 450001,China)

机构地区：[1]河南工业大学生物工程学院,河南郑州450001

出　　处：《河南工业大学学报（自然科学版）》2021年第3期58-63,共6页Journal of Henan University of Technology：Natural Science Edition

基　　金：河南省高校科技创新团队支持计划(19IRTSTHN008)。

摘　　要：酵母甘露聚糖具有多种生物活性,对其生物安全性、体内分布的研究可为体内应用提供基础数据。通过MTT法和体外溶血实验,研究了酵母甘露聚糖(20 k Da)对Caco-2细胞的毒性和红细胞的溶血性,通过对KM实验小鼠灌服100 mg/kg的酵母甘露聚糖溶液,检测酵母甘露聚糖随时间在动物肠道(十二指肠、空肠、回肠)、血液及脏器(心、肝、脾、肺和肾)的分布情况。结果表明:160μg/m L的酵母甘露聚糖对Caco-2细胞无明显毒性,反而有轻微的促增殖作用(115.6%);在80~200μg/m L范围内,酵母甘露聚糖对红细胞的溶血性低于5%,在生物安全范围内;小鼠灌服酵母甘露聚糖后,30~90 min能明显提高血糖水平;1~3 h糖主要分布在肝脏和肺脏中,心脏和肾脏中糖含量变化不大,脾脏糖含量缓慢下降;肠道中未吸收的酵母甘露聚糖,随着时间(0~90 min)推移,在十二指肠中留存时间较短,空肠的糖含量在30 min较高,约60 min时,糖流动到达回肠,酵母甘露聚糖主要在空肠和回肠被吸收。酵母甘露聚糖(20 kDa)有高的生物相容性,无细胞毒性、溶血性,能在小肠中被机体吸收入血,主要分布于肝脏和肺脏中。The mannan produced from Saccharomyces has a variety of biological activities.This study aimed to provide basic data of biosafety and distribution for the applications of the yeast mannan in vivo.The mannan bioactivities,including the cytotoxicity to Caco-2 and the hemolysis to red blood cells,were studied in vitro via the MTT method and hemolysis test.In this study,the molecular weight of mannan was20 k Da.The distribution of the yeast mannan was examined using KMmice administered the solution of yeast mannan with 100 mg/kg BW,then,sugar content was detected over time in the gut of mice(duodenum,jejunum,and ileum),in the blood and the main internal organs(heart,liver,spleen,lung,and kidney).The results showed that the yeast mannan with 20 kDa MW has a great biological safety.The yeast mannan with a concentration of 160μg/mL showed no obvious toxicity to Caco-2 cells,and exerted a slight promotion effect for cell proliferation rate of 115.6%.In the concentration range of 80-200μg/mL,the hemolysis of yeast mannan to erythrocytes was less than 5%,which met the national requirements of biosafety.After the mice were gavaged the solution of yeast mannan for 30-90 min,the blood glucose level was significantly increased.The sugar was mainly distributed in the liver and lung during administration for1 to 3 hours,and the sugar content in the heart and kidney was not changed,while,in the spleen,it decreased slow ly.The residues of yeast mannan unabsorbed in the intestine remained in the duodenum for a short time,then the sugar content in jejunum was higher than that in duodenum and ileum at 30 min.After60 min,the residues of yeast mannan unabsorbed in the intestine flowed to the ileum,yeast mannan was mainly absorbed into the blood in jejunum and ileum during the time of 0 to 90 min.The results of this experiment confirmed that the yeast mannan with 20 kDa MW has high biocompatibility without cytotoxic and hemolytic in vitro,and could be absorbed into the blood in the small intestine,then mainly distributed in the liver

关 键 词：酵母甘露聚糖(20 kDa) 生物安全性 体内分布 细胞毒性 溶血性 

分 类 号：TS201.6[轻工技术与工程—食品科学]