日本结缕草耐逆基因ZjLEA2的克隆及功能探究  

Cloning and Preliminary Function and Analysis of ZjLEA2 from Zoysia japonica

作　　者：龙思欣 农钧绣 王璐 锁忠花 韦善君[1] Long Sixin;Nong Junxiu;Wang Lu;Suo Zhonghua;Wei Shanjun(College of Life and Environmental Science,Minzu University of China,Beijing,100081)

机构地区：[1]中央民族大学生命与环境科学学院,北京100081

出　　处：《基因组学与应用生物学》2021年第1期349-356,共8页Genomics and Applied Biology

基　　金：国家自然科学基金项目(31100570);中央民族大学国家大学生创新训练计划(GCCX2015110020);北京市大学生科学研究与创业行动计划(BEIJ2016110015)共同资助。

摘　　要：植物胚胎发育晚期丰富蛋白(Late embryogenesis abundant)在抵御缺水胁迫中起重要作用。本研究从日本结缕草‘Meyer’品种中克隆获得了1个LEA蛋白基因,命名为ZjLEA2。该基因编码区长501个碱基,推测编码的蛋白质含166个氨基酸,预测分子量17 kD,等电点9.10,具有LEA蛋白家族第二组的典型结构特征,亲水性强。基因的系统发育树分析结果显示,ZjLEA2蛋白与耐旱的禾本科草种狗牙根和无芒隐子草的同源基因遗传距离最近。ZjLEA2基因的表达受干旱、低温和高盐胁迫诱导,表达量峰值均出现在胁迫72 h后。转化ZjLEA2基因的酵母细胞对冷冻低温和高盐胁迫的耐受能力明显增强。本研究结果说明ZjLEA2基因具有抗逆功能,在结缕草抗非生物胁迫中后期起作用。Late embryogenesis abundant(LEA) proteins play key roles in plant under water deficient stress. In this study, a late embryogenesis abundant gene designated ZjLEA2 was cloned from zoysia grass(Zoysia japonica)’Meyer’. The coding sequence of the gene contained 501 bp and was deduced to encode 166 amino acids. The predicted molecular weight and isoelectric point of ZjLEA2 protein were 17 kD and 9.10, respectively. The protein contained typical sequence features were found in the second group of LEA protein and showed strong hydrophilicity.Gene phylogenetic tree analysis indicated that ZjLEA2 protein was closely related to the orthologous genes from two drought tolerance grass species(Cynodon dactylon × Cynodon transvaalensis) and(Cleistogenes songorica). The expression of Zj LEA2 in ’Meyer’ was induced by cold, drought and high salty stresses with the highest level at the72 h-stress point. Yeast cells harboring ZjLEA2 showed increasing tolerance to freezing and salty stress. The results indicated that ZjLEA2 functions during resistance to abiotic stress especially in the middle and later period.

关 键 词：胚胎发育晚期丰富蛋白 结缕草 非生物胁迫 酵母 

分 类 号：S688.4[农业科学—观赏园艺]