Polyherbal formula SC-E3 inhibits rheumatoid arthritis activity in a mouse model of type-Ⅱcollagen-induced arthritis  

作　　者：Ju-Yeon Park Young-Won Kwon Sun-Ah Kim Sun-Dong Park Chang-Hyun Kim Jin-Hee Kim Ju-Hee Lee 

机构地区：[1]College of Korean Medicine,Dongguk University,Goyang 10326,Republic of Korea [2]Department of Medicine,College of Medicine,Dongguk University,Goyang 10326,Republic of Korea [3]Department of Biomedical Laboratory Science,College of Health Science,Cheongju University,Cheongju 28503,Republic of Korea

出　　处：《Journal of Integrative Medicine》2021年第3期265-273,共9页结合医学学报（英文版）

基　　金：supported by the National Research Foundation of Korea grant funded by the Korea government(No.2019R1F1A1062998)。

摘　　要：Objective:SC-E3 is a polyherbal formula that contains five medicinal herbs used frequently in traditional herbal medicine.In our previous study,we demonstrated the antioxidant and anti-inflammatory effects of SC-E3.The present study examined the effects of SC-E3 in a mouse model of type-II collagen-induced arthritis(CIA).Methods:In vivo,male DBA/1 J mice were immunized by intradermal injection of bovine type-II collagen and complete or incomplete Freund’s adjuvant,to induce arthritis.SC-E3 was orally administered daily for 23 days.In vitro,bone marrow-derived macrophages(BMMs)were treated with macrophage colony-stimulating factor(M-CSF)and receptor activator of nuclear factor-j B ligand(RANKL)in the absence or presence of SC-E3.Results:Administrations of SC-E3 were found to have anti-arthritic effects in the joints of CIA mice,as evidenced by reduced paw swelling,bone erosion and deformation,inflammatory cell infiltration,and inflammation in synovial membrane.SC-E3 also reduced serum levels of tumor necrosis factor-a,interleukin-1 b,aspartate aminotransferase and alanine aminotransferase.Furthermore,tartrateresistant acid phosphatase-positive osteoclast numbers in the joints were significantly lower in SC-E3-treated CIA mice than in CIA mice.In addition,the differentiations of BMMs to multinucleated osteoclasts induced by M-CSF and RANKL stimulation were dose-dependently reduced by SC-E3.Conclusion:These results suggest that SC-E3 possesses substantial anti-arthritic activity because it inhibits pro-inflammatory cytokines and osteoclastogenesis,and that SC-E3 has potential therapeutic use for the treatment of rheumatoid arthritis.

关 键 词：Herbal formula ANTI-INFLAMMATORY Rheumatoid arthritis Collagen-induced arthritis OSTEOCLASTOGENESIS 

分 类 号：R285.5[医药卫生—中药学]