AngⅡ及AT1R在膀胱肿瘤发生发展及浸润转移中的意义  

The roles of Ang Ⅱ and AT1R in the development,invasion and metastasis of bladder tumor and their clinical significance

作　　者：涂小峰 李中军 韩新军[1] 胡义 TU Xiao-feng;LI Zhong-jun;HAN Xin-jun;HY Yi(Department of Emergency,Seventh People′s Hospital of ZhengZhou,Zhengzhou 450016,Henan,China;不详)

机构地区：[1]郑州市第七人民医院急诊科,河南郑州450016 [2]河南省人民医院泌尿外科,河南郑州450003

出　　处：《广东医学》2021年第6期661-665,共5页Guangdong Medical Journal

基　　金：河南省医学科技攻关计划项目(2016R4136)。

摘　　要：目的检测血管紧张素Ⅱ(angiotensin Ⅱ,Ang Ⅱ)及血管紧张素Ⅱ1型受体(angiotensin Ⅱ 1 receptor,AT1R)在膀胱癌中的表达情况,并探讨其在膀胱癌进展中的临床意义。方法分别采用免疫组化方法和荧光定量RT-PCR检测60例膀胱癌组织及配对旁癌组织中Ang Ⅱ及AT1R蛋白和mRNA的表达情况。分析Ang Ⅱ及AT1R与膀胱癌三者在膀胱癌分化程度、浸润程度及淋巴结转移等临床病理特征的相关性。并通过多因素logistic回归分析Ang Ⅱ及AT1R的影响因素。结果60例膀胱癌组织中,Ang Ⅱ及AT1R阳性表达率分别为65.00%、68.33%。膀胱癌组织Ang Ⅱ的mRNA相对表达量为1.89±0.51,癌旁组织的为1.06±0.25,膀胱癌组织AT1R mRNA相对表达量为1.95±0.53,癌旁组织的为1.04±0.21,差异有统计学意义(P<0.05)。Ang Ⅱ及AT1R阳性表达率随着肿瘤分化程度、浸润深度、淋巴结转移、TNM分期的增加而增加,多因素logistic回归分析结果显示,膀胱癌患者的分化程度、浸润深度、肿瘤分期和淋巴结转移会明显影响Ang Ⅱ及AT1R的表达,差异均有统计学意义(P<0.05)。结论膀胱癌组织Ang Ⅱ及AT1R表达与发生发展、浸润程度和淋巴结远处转移明显相关,Ang Ⅱ及AT1R可能成为预测膀胱癌进展、浸润和转移的分子标记物。Objective To assess the expression of Ang Ⅱ and AT1R in bladder cancer and explore its clinical significance in the progression of bladder cancer.Methods Immunohistochemistry and fluorescence quantitative RT-PCR were used to measure the expression of Ang Ⅱ and AT1R protein and mRNA in 60 cases of bladder cancer tissues and paired adjacent cancer tissues.The correlations between Ang Ⅱ ,AT1R and clinicopathological characteristics,such as the degree of differentiation,infiltration and lymph node metastasis of bladder cancer,were analyzed.Through multi-factor logistics regression analysis,the influencing factors of Ang Ⅱ and AT1R proteins were analyzed.Results In 60 cases of bladder cancer,the positive rates of Ang Ⅱ and AT1R were 65.00%and 68.33%,respectively.The relative expression of Ang Ⅱ mRNA in bladder cancer tissue was 1.89±0.51,and in the adjacent tissue was 1.06±0.25;the relative expression of AT1R mRNA in bladder cancer tissue was 1.95±0.53,and in the adjacent tissue was 1.04±0.21;the difference was statistically significantly(P<0.05).The positive expression rate of Ang Ⅱ and AT1R increased with the degree of tumor differentiation,depth of invasion,lymph node metastasis,and TNM stage.Multivariate logistics regression analysis showed that the degree of differentiation,depth of invasion,tumor stage,lymph node metastasis and lymph node metastasis stage significantly affected the expression of Ang Ⅱ and AT1R protein(P<0.05).Conclusion The expression of Ang Ⅱ and AT1R in bladder cancer tissue is correlated to the occurrence and development,the degree of invasion and the distant metastasis of lymph nodes in bladder cancer.Ang Ⅱ and AT1R may become molecular markers in predicting the progression,invasion and metastasis of bladder cancer.

关 键 词：血管紧张素Ⅱ 血管紧张素Ⅱ1型受体 膀胱癌 浸润 转移 

分 类 号：R737.14[医药卫生—肿瘤] R446[医药卫生—临床医学]