基于Er^(3+)掺杂上转换纳米粒子的生物成像研究进展  

Advances of Er^(3+) Doped Upconversion Nanoparticles for Biological Imaging

作　　者：彭皓 杨方 杜慧 姜波 姚晨阳 姚俊列 郑方 吴爱国 PENG Hao;YANG Fang;DU Hui;JIANG Bo;YAO Chen-Yang;YAO Jun-Lie;ZHENG Fang;WU Ai-Guo(Cixi Institute of Biomedical Engineering,Ningbo Institute of Materials Technology and Engineering,Chinese Academy of Sciences(CAS),CAS Key Laboratory of Magnetic Materials and Devices&Zhejiang Engineering Research Center for Biomedical Materials,Ningbo 315201,China)

机构地区：[1]中国科学院宁波材料技术与工程研究所慈溪生物医学工程研究所,中国科学院磁性材料与器件重点实验室,医用植介入材料浙江省工程研究中心,宁波315201

出　　处：《分析化学》2021年第7期1106-1120,共15页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金项目(Nos.32025021,31971292,51873225,51803228);科技部项目(Nos.2018YFC0910601,SQ2019YFA040008-03)资助。

摘　　要：上转换纳米粒子能够在近红外光的激发下发射可见光波段的高能光子。相对于传统荧光材料,上转换纳米粒子具有更好的组织穿透深度以及更好的生物相容性,减少了生物细胞组织自身荧光干扰,在高灵敏度的生物成像领域具有广阔的应用前景。铒(Erbium,Er)作为一种稀土金属元素,在近红外Ⅱ区(1500 nm)有很强的吸收,同时发射出红光和绿光,是一种光学性能优良的材料,通常被掺杂在上转换纳米粒子中作为发光中心。然而,Er^(3+)掺杂的上转换纳米粒子由于表面猝灭、能量逆流等问题,限制了其发光效率和成像效果。本文对Er^(3+)掺杂的上转换纳米粒子材料在性能优化以及生物成像应用方面的应用研究进行了总结,并对其在时域通路成像领域的最新进展进行了讨论,对其发展前景展望。Upconversion nanoparticles can emit high-energy photons in visible region under the excitation of near-infrared light.Compared with traditional fluorescent materials,upconversion nanoparticles show better tissue penetration depth and biocompatibility,and also reduce the fluorescence interference of biological cells and tissues.Therefore,upconversion nanoparticles have broad application prospects in the field of highly sensitivie biological imaging.Erbium(Er)has a strong absorption in the near infraredⅡregion(1500 nm),and can emit red or green light.With the excellent optical properties,Erbium is usually doped in the upconversion nanoparticles as the emission center.However,due to the problems of surface quenching and energy countercurrent,the upconversion nanoparticles doped with Er^(3+)have limited luminescence efficiency and low biological imaging performance.In this review,the development of Er^(3+)doped upconversion nanoparticles in optical properties optimization and biological imaging applications are summarized,and the latest progress and development prospects of Er^(3+)doped upconversion nanoparticle materials in the domain of temporal pathway imaging are discussed and prospected.

关 键 词：上转换发光 铒离子掺杂 生物成像 多模态图像 时域光谱 评述 

分 类 号：Q-334[生物学] TB383.1[一般工业技术—材料科学与工程]