Synergistic anti-inflammatory and osteogenic n-HA/resveratrol/chitosan composite microspheres for osteoporotic bone regeneration  

作　　者：Limei Li Mali Yu Yao Li Qing Li Hongcai Yang Meng Zheng Yi Han Di Lu Sheng Lu Li Gui 

机构地区：[1]Yunnan Key Laboratory of Stem Cell and Regenerative Medicine,Science and Technology Achievement Incubation Center,Kunming Medical University,Kunming,650500,China [2]Department of Stomatology,The First People’s Hospital of Yunnan Province,Kunming,650032,China [3]Department of Neurology,The First Affiliated Hospital,Kunming Medical University,Kunming,650000,China [4]Yunnan Key Laboratory of Digital Orthopaedics,Department of Orthopaedics,The First People’s Hospital of Yunnan Province,Kunming,650032,China [5]Department of Endocrinology,The Third People’s Hospital of Yunnan Province,Kunming,650011,China

出　　处：《Bioactive Materials》2021年第5期1255-1266,共12页生物活性材料（英文）

基　　金：supported by the National Natural Science Foundation of China(81460173/81860326/81960268);the Department of Science and Technology of Yunnan Province of China(2017FF117(-062)/2018FE001(-137)/2018FE001(-125)/2019ZF011-2).

摘　　要：The development of functional materials for osteoporosis is ultimately required for bone remodeling.However,grafts were accompanied by increasing pro-inflammatory cytokines that impaired bone formation.In this work,nano-hydroxyapatite(n-HA)/resveratrol(Res)/chitosan(CS)composite microspheres were designed to create a beneficial microenvironment and help improve the osteogenesis by local sustained release of Res.Study of in vitro release confirmed the feasibility of n-HA/Res/CS microspheres for controlled Res release.Notably,microspheres had anti-inflammatory activity evidenced by the decreased expression of pro-inflammatory cytokines TNF-α,IL-1βand iNOS in RAW264.7 cells in a dose dependent manner.Further,enhanced adhesion and proliferation of BMSCs seeded onto microspheres demonstrated that composite microspheres were conducive to cell growth.The ability to enhance osteo-differentiation was supported by up-regulation of Runx2,ALP,Col-1 and OCN,and substantial mineralization in osteogenic medium.When implanted into bone defects in the osteoporotic rat femoral condyles,enhanced entochondrostosis and bone regeneration suggested that the n-HA/Res/CS composite microspheres were more favorable for impaired fracture healing.The results indicated that optimized n-HA/Res/CS composite microspheres could serve as promising multifunctional fillers for osteoporotic bone defect/fracture treatment.

关 键 词：n-HA/Res/CS microsphere ANTI-INFLAMMATION Osteo-differentiation Osteoporotic bone regeneration 

分 类 号：R318[医药卫生—生物医学工程]