Microbial soluble aromatic prenyltransferases for engineered biosynthesis  

作　　者：He-Ping Chen Ikuro Abe 

机构地区：[1]Graduate School of Pharmaceutical Sciences,The University of Tokyo,Bunkyo-ku,Tokyo,113-0033,Japan [2]School of Pharmaceutical Sciences,South-Central University for Nationalities,Wuhan,Hubei,430074,PR China [3]Collaborative Research Institute for Innovative Microbiology,The University of Tokyo,Yayoi 1-1-1,Bunkyo-ku,Tokyo,113-8657,Japan

出　　处：《Synthetic and Systems Biotechnology》2021年第2期51-62,共12页合成和系统生物技术（英文）

基　　金：supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education,Culture,Sports,Science and Technology,Japan(JSPS KAKENHI Grant No.JP16H06443 and JP20H00490);Japan Science and Technology Agency(JST SICORP Grant No.JPMJSC1701);H.P.C.is a recipient of the JSPS Postdoctoral Fellowship for Foreign Researchers(ID No.P19413).

摘　　要：Prenyltransferase(PTase)enzymes play crucial roles in natural product biosynthesis by transferring isoprene unit(s)to target substrates,thereby generating prenylated compounds.The prenylation step leads to a diverse group of natural products with improved membrane affinity and enhanced bioactivity,as compared to the nonprenylated forms.The last two decades have witnessed increasing studies on the identification,characterization,enzyme engineering,and synthetic biology of microbial PTase family enzymes.We herein summarize several examples of microbial soluble aromatic PTases for chemoenzymatic syntheses of unnatural novel prenylated compounds.

关 键 词：Microbial prenyltransferase BIOSYNTHESIS Enzyme engineering PRENYLATION Synthetic biology 

分 类 号：O62[理学—有机化学]