消积抑癌方对Lewis肺癌荷瘤小鼠MMP-2的影响  

Effect of Xiaoji Yi’ai recipe on MMP-2 in Lewis lung cancer bearing mice

作　　者：郑茜 刘远婷 丁甜甜 李慧 ZHENG Qian;LIU Yuanting;DING Tiantian;LI Hui(Department of Traditional Chinese Medicine,the Seventh Affiliated Hospital of Xinjiang Medical University,Urumqi 830000,China)

机构地区：[1]新疆医科大学第七附属医院中医科,新疆乌鲁木齐830000

出　　处：《陕西中医》2021年第7期844-848,共5页Shaanxi Journal of Traditional Chinese Medicine

基　　金：新疆维吾尔自治区自然科学基金资助项目(2018D01C242)。

摘　　要：目的:探讨消积抑癌方对Lewis肺癌荷瘤小鼠MMP-2的影响。方法:建立Lewis肺癌荷瘤小鼠模型,随机分为空白组、荷瘤组、消积抑癌方内服组、消积抑癌方外敷组、消积抑癌方内服联合外敷组。观察各组Lewis肺癌荷瘤小鼠肿瘤体积、癌重、抑癌率,对比各组免疫器官重量及血清基质金属蛋白酶-2(MMP-2)、血管内皮生长因子(VEGF)、缺氧诱导因子-1α(HIF-1α)、干扰素(IFN)-γ、IL-10、TNF-α、IL-2水平。结果:第7天、第14天、第21天及第30天,联合组Lewis肺癌荷瘤小鼠肿瘤体积、癌重、抑癌率均明显低于荷瘤组、内服组、外敷组;内服组、外敷组Lewis肺癌荷瘤小鼠肿瘤体积、癌重、抑癌率均明显低于荷瘤组,差异具有统计学意义(P<0.05);空白组、联合组脾脏指数、胸腺指数与荷瘤组、内服组、外敷组比较,差异有统计学意义(P<0.05);空白组、联合组MMP-2、VEGF、HIF-1α、IFN-γ、IL-10、TNF-α、IL-2水平比较,差异有统计学意义(P<0.05);内服组、外敷组MMP-2、VEGF、HIF-1α、IFN-γ、IL-10、TNF-α、IL-2水平与荷瘤组、空白组比较,差异有统计学意义(P<0.05);荷瘤组MMP-2、VEGF、HIF-1α、IFN-γ、IL-10、TNF-α、IL-2水平与空白组比较,差异有统计学意义(P<0.05)。结论:消积抑癌方内服联合外敷能够有效抑制Lewis肺癌荷瘤小鼠体内肿瘤,降低MMP-2水平,调节机体免疫功能。Objective:To explore the effect of Xiaoji Yi’ai recipe on MMP-2 in mice bearing Lewis lung cancer.Methods:Lewis lung cancer tumor-bearing mouse model were established and randomly divided into a blank group,a tumor-bearing group,Xiaoji Yi’ai recipe orally taken group,Xiaoji Yi’ai recipe external application group,Xiaoji Yi’ai recipe orally combined with external application group.Observed the tumor volume,cancer weight and tumor suppressor rate of each group of Lewis lung cancer tumor-bearing mice,compare the weight of immune organs and MMP-2,VEGF,and HIF-1α,IFN-γ,IL-10,TNF-α,IL-2 levels.Results:On day 7,14,21 and 30,the tumor volume,cancer weight,and tumor suppression rate of Lewis lung cancer bearing mice in the combined group were significantly lower than those in the tumor-bearing group,oral administration group,and external application group.The tumor volume,cancer weight and tumor suppression rate of Lewis lung cancer bearing mice in the external application group were significantly lower than those in the tumor bearing group,and the difference was statistically significant(P<0.05).The spleen index and thymus index of the blank group and the combined group were compared with the tumor-bearing group,the oral administration group,and the external application group,and the difference was statistically significant(P<0.05),the spleen index and thymus index of the internal administration group,the external application group and the tumor-bearing group and the blank group The difference was statistically significant(P<0.05).the spleen index and thymus index of the tumor-bearing group were compared with the blank group,and the difference was statistically significant(P<0.05).Comparison of the levels of MMP-2,VEGF,HIF-1α,IFN-γ,IL-10,TNF-α,IL-2 between the blank group and the combination group was statistically significant(P<0.05).the MMP of the oral and external application group MMP-2,VEGF,HIF-1α,IFN-γ,IL-10,TNF-α,IL-2 levels compared with tumor-bearing group and blank group,the difference was stat

关 键 词：肺癌 消积抑癌方 小鼠 肿瘤 血清基质金属蛋白酶-2 免疫功能 

分 类 号：R734.2[医药卫生—肿瘤]