Injectable silk sericin scaffolds with programmable shape-memory property and neuro-differentiation-promoting activity for individualized brain repair of severe ischemic stroke  

作　　者：Jian Wang Xiaolin Li Yu Song Qiangfei Su Xiakeerzhati Xiaohalati Wen Yang Luming Xu Bo Cai Guobin Wang Zheng Wang Lin Wang 

机构地区：[1]Department of Clinical Laboratory,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan,430022,China [2]Research Center for Tissue Engineering and Regenerative Medicine,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan,430022,China [3]Department of Gastrointestinal Surgery,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan,430022,China

出　　处：《Bioactive Materials》2021年第7期1988-1999,共12页生物活性材料（英文）

基　　金：This work was supported by the National Key Basic Research Program of China[2015CB5540007];the National Natural Science Foundation of China[81671904,81572866,81773104,and 81873931];the Science and Technology Program of Chinese Ministry of Education[113044A];the Major Scientific and Technological Innovation Projects in Hubei Province[2018ACA136];the Integrated Innovative Team for Major Human Diseases Program of Tongji Medical College of HUST;the Academic Doctor Supporting Program of Tongji Medical College of HUST.

摘　　要：Severe ischemic stroke damages neuronal tissue,forming irregular-shaped stroke cavities devoid of supporting structure.Implanting biomaterials to provide structural and functional support is thought to favor ingrowth of regenerated neuronal networks.Injectable hydrogels capable of in situ gelation are often utilized for stroke repair,but challenged by incomplete gelation and imprecise control over end-macrostructure.Injectable shape-memory scaffolds might overcome these limitations,but are not explored for stroke repair.Here,we report an injectable,photoluminescent,carbon-nanotubes-doped sericin scaffold(CNTs-SS)with programmable shape-memory property.By adjusting CNTs’concentrations,CNTs-SS′recovery dynamics can be mathematically calculated at the scale of seconds,and its shapes can be pre-designed to precisely match any irregular-shaped cavities.Using a preclinical stroke model,we show that CNTs-SS with the customized shape is successfully injected into the cavity and recovers its pre-designed shape to well fit the cavity.Notably,CNTs-SS’near-infrared photoluminescence enables non-invasive,real-time tracking after in vivo implantation.Moreover,as a cell carrier,CNTs-SS not only deliver bone marrow mesenchymal stem cells(BMSCs)into brain tissues,but also functionally promote their neuronal differentiation.Together,we for the first time demonstrate the feasibility of applying injectable shape-memory scaffolds for stroke repair,paving the way for personalized stroke repair.

关 键 词：Stroke cavities Silk sericin scaffolds Shape-memory properties INJECTABILITY Neuronal differentiation 

分 类 号：R743.3[医药卫生—神经病学与精神病学]