Analysis of structural components of decellularized scaffolds in renal fibrosis  

作　　者：Rui Zhang Junqun Jiang Yaling Yu Fangfang Wang Niuniu Gao Yingjie Zhou Xinlong Wan Zhibin Wang Peng Wei Jin Mei 

机构地区：[1]Institute of Bioscaffold Transplantation and Immunology,Wenzhou Medical University,North Center Road,Ouhai District,Wenzhou,325035,China [2]Institute of Biomaterials,Ningbo City First Hospital,No.59 Liuting Street,Haishu District,Ningbo,315010,China [3]Department of Orthopaedics,Shanghai Jiao Tong University Affiliated Sixth People’s Hospital,No.600 Yishan Road,Shanghai,200233,China

出　　处：《Bioactive Materials》2021年第7期2187-2197,共11页生物活性材料（英文）

基　　金：The authors would like to acknowledge the Institute of Bioscaffold Transplantation and Immunology whose research was funded by the National Natural Science Foundation of China(grant NO.81970653);And the Medical Research Center of Ningbo City First Hospital whose research was funded by Zhejiang Medical and Health Technology Project(grant No.2018248656);Zhejiang pharmacy Top Key Project(grant No.YKFJ2-008).

摘　　要：Chronic kidney disease has been recognized as a major public health problem worldwide and renal fibrosis is a common pathological process occurring in chronic renal failure.It is very promising to find the strategies to slow or even prevent the progression of fibrosis.This study focused on whether renal fibrosis decellularized scaffolds has the potential to be a model of cellular mechanisms of tissue fibrosis or donors for tissue engineering.In order to evaluate the feasibility of decellularized scaffolds derived from pathological kidneys,histology,proteomics and ELISA will be used to analysis the changes in the structure and main components of fibrotic tissue.The fibrosis model in this paper was induced by adenine-fed and the results showed that the structure of fibrotic scaffold was changed and some protein were up-regulated or down-regulated,but the cytokines associated with renal regeneration after injury were remained.In cell experiments,endothelial progenitor cells proliferated well,which proved that the fibrotic scaffolds have non-cytotoxic.All these conclusions indicate that the renal fibrosis decellularized scaffolds model has the ability to study fibrosis mechanism and the potential to be engineering donors as well as normal scaffolds.

关 键 词：Extracellular matrix PROTEOMICS Growth factor Tissue engineering Chronic renal diseases 

分 类 号：R318[医药卫生—生物医学工程]