Exosome-functionalized polyetheretherketone-based implant with immunomodulatory property for enhancing osseointegration  

作　　者：Lei Fan Pengfei Guan Cairong Xiao Huiquan Wen Qiyou Wang Can Liu Yian Luo Limin Ma Guoxin Tan Peng Yu Lei Zhou Chengyun Ning 

机构地区：[1]School of Materials Science and Engineering&National Engineering Research Center for Tissue Restoration and Reconstruction,South China University of Technology,Guangzhou,510641,China [2]Department of Orthopedic Surgery,Nanfang Hospital,Southern Medical University,Guangzhou,510515,China [3]Department of Spine Surgery,the Third Affiliated Hospital of Sun Yat-sen University,Guangzhou,510630,China [4]School of Chemical Engineering and Light Industry,Guangdong University of Technology,Guangzhou,510006,China [5]Department of Radiology,the Third Affiliated Hospital of Sun Yat-Sen University,Guangzhou,510630,China [6]Department of Spine Surgery,the First Hospital of Zhejiang University,Hangzhou,310003,China [7]Department of Orthopedics,Guangdong Provincial People’s Hospital,Guangdong Academy of Medical Sciences,Guangzhou,510080,China

出　　处：《Bioactive Materials》2021年第9期2754-2766,共13页生物活性材料（英文）

基　　金：This work was supported by the the National Natural Science Foundation of China(Nos.51932002,51903087,51772106,31771080);the Natural Science Foundation of Guangdong Province(No.2020A1515011369);the Science and Technology Program of Guangzhou(No.202002030308);the Science and Technology Innovation Team Project of Foshan(No.2018IT100101);Sino-Singapore International Joint Research Institute(No.203-A018004).

摘　　要：The host immune response effecting on biomaterials is critical to determine implant fates and bone regeneration property.Bone marrow stem cells(BMSCs)derived exosomes(Exos)contain multiple biosignal molecules and have been demonstrated to exhibit immunomodulatory functions.Herein,we develop a BMSC-derived Exos-functionalized implant to accelerate bone integration by immunoregulation.BMSC-derived Exos were reversibly incorporated on tannic acid(TA)modified sulfonated polyetheretherketone(SPEEK)via the strong interaction of TA with biomacromolecules.The slowly released Exos from SPEEK can be phagocytosed by co-cultured cells,which could efficiently improve the biocompatibilities of SPEEK.In vitro results showed the Exos loaded SPEEK promoted macrophage M2 polarization via the NF-κB pathway to enhance BMSCs osteogenic differentiation.Further in vivo rat air-pouch model and rat femoral drilling model assessment of Exos loaded SPEEK revealed efficient macrophage M2 polarization,desirable new bone formation,and satisfactory osseointegration.Thus,BMSC-derived Exos-functionalized implant exerted osteoimmunomodulation effect to promote osteogenesis.

关 键 词：OSTEOIMMUNOLOGY Macrophages polarization POLYETHERETHERKETONE BMSC-Derived exosomes Bone regeneration 

分 类 号：R318[医药卫生—生物医学工程]