Simple application of adipose-derived stem cell-derived extracellular vesicles coating enhances cytocompatibility and osteoinductivity of titanium implant  

作　　者：Lifeng Chen Shan Mou Jinfei Hou Huimin Fang Yuyang Zeng Jiaming Sun Zhenxing Wang 

机构地区：[1]Department of Plastic Surgery,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China [2]Wuhan Clinical Research Center for Superficial Organ Reconstruction,Wuhan Union Hos pital,Huazhong University of Science and Technology,1277 Jiefang Avenue,Wuhan 430022,China

出　　处：《Regenerative Biomaterials》2021年第1期64-72,共9页再生生物材料（英文版）

基　　金：This work was supported by the National Key R&D Program of China(2019YFA0110500);the National Natural Science Foundation of China(No.81873941,81701922 and 81701912).

摘　　要：Surface modification using bioactive molecules is frequently performed to improve the biological properties of medical metal biomaterial titanium(Ti)implants.Developmental evidence suggests that mesenchymal stem cell-derived extracellular vesicles(MSC-EVs)served as potent bioactive component.As a subset of MSC EV,adipose-derived stem cel-derived extracellular vesicles(ADSC-EVs)could be obtained from abundant adipose tissue.Meanwhile,it possesses multiple re-generative properties and might be used to endow biological activities to medical Ti implant.Here,we present a simple ADSC-EV coating strategy based on physisorption of fibronectin.This ADSC-EV functionalized Ti implants(EV-Ti)revealed enhanced osteoblast compatibility and osteoinduc-tive activity.Cell spreading area of EV-Ti group was 1.62-and 1.48-fold larger than that of Ti group after 6 and 12 h of cell seeding,respectively.Moreover,EV-Ti promoted alkaline phosphatase,col-lagen 1 and osteocalcin gene expression in osteoblast by 1.51-,1.68-and 1.82-fold compared with pristine Ti,respectively.Thus,the MSC-EVs modification method reported here provide a clinically translatable strategy to promote the bioactivity of Ti implants.

关 键 词：adipose-derived stem cll-derived extraellular vesicles titanium surface modification FIBRONECTIN OSTEOINDUCTIVITY 

分 类 号：R318[医药卫生—生物医学工程]