Evaluating the potential of an amelogenin-derived peptide in tertiary dentin formation  

作　　者：Xiu Peng Sili Han Kun Wang Longjiang Ding Zhenqi Liu Linglin Zhang 

机构地区：[1]State Key Laboratory of Oral Diseases,Department of Cariology and Endodontics,National Clinical Research Center for Oral Diseases,West China Hospital of Stomatology,Sichuan University,Chengdu,China

出　　处：《Regenerative Biomaterials》2021年第2期85-93,共9页再生生物材料（英文版）

基　　金：This work was supported by the National Natural Science Foundation of China(grants number 81771062 and 81970931).

摘　　要：Several novel biomaterials have been developed for dental pulp capping by inducing tertiary dentin formation.The aim of this study was to evaluate the effect of QP5,an amelogenin-based peptide,on the mineralization of dental pulp cells(DPCs)in vitro and in vivo.The cell viability of human DPCs(hDPCs)after treatment with QP5 was determined using the Cell Counting Kit-8(CCK-8).Migration of hDPCs was assessed using scratch assays,and the pro-mineralization effect was determined using alkaline phosphatase(ALP)staining,alizarin red staining and the expression of mineralization-related genes and proteins.The results showed that QP5 had little effect on the cell viability,and significantly enhanced the migration capability of hDPCs.QP5 promoted the formation of mineralized nodules,and upregulated the activity of ALP,the expression of mRNA and proteins of mineralization-related genes.A pulp capping model in rats was generated to investigate the biological effect of QP5.The results of micro-computed tomography and haematoxylin and eosin staining indicated that the formation of tertiary dentin in QP5-capping groups was more prominent than that in the negative control group.These results indicated the potential of QP5 as a pulp therapy agent.

关 键 词：tertiary dentin amelogenin-derived peptide pulp capping dental pulp cells 

分 类 号：R78[医药卫生—口腔医学]