1型糖尿病早期大鼠空肠的神经功能及组织形态学变化  

Jejunal Nerve Regulation and Histomorphological Changes in Rats with Early Type 1 Diabetes

作　　者：王嫚 祗明花 耿旭芳 佟苗苗[1] 梁红玉[1] 赵丁[1] WANG Man;ZHI Minghua;GENG Xufang;TONG Miaomiao;LIANG Hongyu;ZHAO Ding(School of Pharmacy,Hebei Medical University,Shijiazhuang 050017,China)

机构地区：[1]河北医科大学药学院,石家庄050017

出　　处：《实验动物与比较医学》2021年第2期131-137,共7页Laboratory Animal and Comparative Medicine

基　　金：河北省重点基础研究项目(17967753D)。

摘　　要：目的探讨1型糖尿病早期大鼠空肠平滑肌的氮能神经和胆碱能神经功能,以及空肠组织形态学变化。方法将雄性S D大鼠随机分为正常对照组和糖尿病模型组,通过腹腔注射链脲佐菌素(streptozocin,STZ)60 mg/kg建立糖尿病大鼠模型。4周后运用电场刺激诱发神经源性收缩反应,并采用离体器官肌张力分析方法观察离体空肠平滑肌的收缩反应,以及氮能神经和胆碱能神经功能的改变;石蜡组织切片行H E染色及免疫组织化学检测,观察空肠组织形态学变化。结果与正常大鼠相比,STZ诱导的糖尿病早期大鼠的空肠肌间胆碱能神经功能受损,而氮能神经功能未见明显变化;糖尿病大鼠空肠组织绒毛杂乱无序且断裂,肌层增厚,杯状细胞减少,绒毛长度与肠隐窝深度之比(绒腺比)减小。结论在STZ诱导后4周的1型糖尿病早期大鼠模型中,空肠组织的神经功能和形态学均发生了明显变化,提示肠道作为糖尿病敏感器官,在糖尿病早期诊断及预防中具有重要的临床参考意义。Objective To investigate functional changes of nitrergic and cholinergic nerves in the jejunum smooth muscle, and to observe jejunal histomorphology in rats with early type 1 diabetes. Methods Male Sprague Dawley(SD) rats were randomly divided into a normal control group and a diabetic model group.Rats in the diabetic model group were administered 60 mg/kg streptozotocin(STZ) by intraperitoneal injection.After 4 weeks, neurogenic contractile responses were induced by electric field stimulation, and functional changes in nitrergic and cholinergic nerves were observed in the isolated jejunum of diabetic rats.Histomorphological changes in the jejunum were observed by hematoxylin and eosin staining of paraffin-embedded tissue sections and immunohistochemistry. Results Compared with that in the normal rats, the jejunal intermuscular cholinergic nerve rats with early diabetes was damaged, whereas the nitrogenous nerve did not exhibit a such lesions. The villi of the jejunum were disrupted and disorderly, the muscularis was thickened, goblet cell numbers were decreased, and the ratio of villus length to intestinal crypt depth was diminished in diabetic rats. Conclusion In the early stage(4 weeks) of STZ-induced type 1 diabetes in rats, the neurological function and morphology of jejunal tissues were significantly changed. It suggests that the intestine is a sensitive organ in diabetes and has important clinical implications for the early prevention and detection of diabetes.

关 键 词：糖尿病大鼠 空肠 氮能神经 胆碱能神经 组织形态学 

分 类 号：R-332[医药卫生] Q95-33[生物学—动物学]