固定化脂肪酶催化合成生物基增塑剂2,5-呋喃二甲酸正丁酯  

Synthesis of bio-based plasticizer dibutyl furan-2,5-dicarboxylate catalyzed by immobilized lipase

作　　者：杜雪飞 李雪玉 郭淑怡 贾红华[1] 周华[1] DU Xuefei;LI Xueyu;GUO Shuyi;JIA Honghua;ZHOU Hua(College of Biotechnology and Pharmaceutical Engineering,Nanjing TECH University,Nanjing 211816,Jiangsu,China)

机构地区：[1]南京工业大学生物与制药工程学院,江苏南京211816

出　　处：《精细化工》2021年第6期1192-1198,共7页Fine Chemicals

基　　金：国家重点基础研究计划(973)项目(2013CB733904)。

摘　　要：以固定化脂肪酶Novozym435为催化剂,在甲苯中催化2,5-呋喃二甲酸二甲酯与正丁醇转酯化反应制备2,5-呋喃二甲酸正丁酯(DBF),对酶促合成DBF的工艺进行优化,探讨了糖类物质和吸附剂作为添加剂对DBF收率的影响。结果表明,在n(2,5-呋喃二甲酸二甲酯)∶n(正丁醇)=1∶5,其中,2,5-呋喃二甲酸二甲酯的物质的量为1 mmol,固定化脂肪酶Novozym435添加量为925 U的10 mL甲苯中,45℃、150 r/min反应28 h后,DBF的收率达到最大。4A分子筛的加入能明显促进DBF的生成,海藻糖的加入也增强了反应体系中脂肪酶的催化能力,在反应体系中加入0.2 kg/L 4A分子筛和固定化脂肪酶质量15%的海藻糖,45℃反应14 h,DBF的收率达到了87.25%,固定化脂肪酶Novozym435在DBF的最优合成体系中连续使用10次后,DBF的收率仍然能保持在47.68%。Dibutyl furan-2,5-dicarboxylate(DBF) was prepared by transesterification method of dimethyl 2,5-furandicarboxylate with butanol using toluene as solvent and immobilized lipase Novozym435 as catalyst. The synthesis process of DBF was optimized. The effects of sugars and adsorbents as additives on the yield of DBF were discussed. The results showed that under the conditions of n(dimethyl 2,5-furandicarboxylate)∶n(butanol)=1∶5, n(dimethyl 2,5-furandicarboxylate) = 1 mmol, Novozym435 enzyme dosage 925 U, reaction temperature 45 ℃, stirring speed 150 r/min, 10 mL toluene as solvent, and reaction time 28 h, the yield of DBF was the highest. The addition of 4 A molecular sieve could obviously promote the formation of DBF, and the addition of trehalose could also enhance the catalytic ability of lipase. The yield of DBF reached 87.25% after adding 0.2 kg/L 4 A molecular sieve and trehalose dosage being 15% of immobilized lipase mass into the reaction system and reacting at 45 ℃ for 14 h. The yield of DBF was still 47.68% after 10 times of immobilized lipase Novozym435 continuous use in the optimal DBF synthesis system.

关 键 词：增塑剂 脂肪酶 2 5-呋喃二甲酸正丁酯 工艺优化 分子筛 海藻糖 催化技术 

分 类 号：TQ426.97[化学工程] TQ225.2