甲醇酵母代谢工程研究进展  

Advances in metabolic engineering of methylotrophic yeasts

作　　者：高琳惠 蔡鹏 周雍进[1,2] Linhui Gao;Peng Cai;Yongjin J.Zhou(Division of Biotechnology,Dalian Institute of Chemical Physics,Chinese Academy of Sciences,Dalian 116023,Liaoning,China;Dalian Key Laboratory of Energy Biotechnology,Dalian Institute of Chemical Physics,Chinese Academy of Sciences,Dalian 116023,Liaoning,China)

机构地区：[1]中国科学院大连化学物理研究所生物技术研究部,辽宁大连116023 [2]大连市能源生物技术重点实验室,辽宁大连116023

出　　处：《生物工程学报》2021年第3期966-979,共14页Chinese Journal of Biotechnology

基　　金：国家自然科学基金(Nos.21922812,31700082);兴辽英才计划(No.XLYC1807191)资助。

摘　　要：甲醇酵母由于独特优点被认为是绿色生物制造的潜在宿主。特别是其天然甲醇利用性能有望建立甲醇生物转化路线,拓展生物炼制底物,具有重要经济价值和环保意义。文中综述了代谢工程改造甲醇酵母合成蛋白质和化学品的最新研究进展,并比较了其与模式生物酿酒酵母作为细胞工厂的优缺点。随后,分析了甲醇酵母代谢工程改造面临的挑战,并展望了潜在解决方案。随着基因操作工具开发和细胞代谢阐释,甲醇酵母将在未来绿色生物制造发挥越来越重要的作用。Methylotrophic yeasts are considered as promising cell factories for bio-manufacturing due to their several advantages such as tolerance to low p H and high temperature.In particular,their methanol utilization ability may help to establish a methanol biotransformation process,which will expand the substrate resource for bio-refinery and the product portfolio from methanol.This review summarize current progress on engineering methylotrophic yeasts for production of proteins and chemicals,and compare the strengths and weaknesses with the model yeast Saccharomyces cerevisiae.The challenges and possible solutions in metabolic engineering of methylotrophic yeasts are also discussed.With the developing efficient genetic tools and systems biology,the methylotrophic yeasts should play more important roles in future green bio-manufacturing.

关 键 词：绿色生物制造 代谢工程 合成生物学 基因编辑 甲醇生物转化 

分 类 号：Q78[生物学—分子生物学] TQ920.1[轻工技术与工程—发酵工程]