海带多糖通过促进Sirt3/MnSOD表达来改善受照射后小鼠的下颌下腺损伤  

Laminaria Japonica Polysaccharide attenuates radiation-induced submandibular gland injury in mice via promoting Sirt3/MnSOD expression

作　　者：张思琴 敖翩 陈少勇 柴溶 王绍军[2] 韦力[3] 徐杨 陈晨 ZHANG Siqin;AO Pian;CHEN Shaoyong;CHAI Rong;WANG Shaojun;WEI Li;XU Yang;CHEN Chen(College of Stomatology,Guangxi Medical University,Nanning 530021;Materna and Child Health Hospital of Guangxi Zhuang Autonomous Region,Nanning 530021;Department of Human Anatomy,Guangxi Medical University,Nanning 530021)

机构地区：[1]广西医科大学口腔医学院,南宁530021 [2]广西壮族自治区妇幼保健院,南宁530021 [3]广西医科大学人体解剖学教研室,南宁530021

出　　处：《辐射防护》2021年第3期264-270,共7页Radiation Protection

基　　金：国家自然科学基金项目(81460479);广西自然科学基金项目(2018JJA140600);广西教育厅基金(ZD2014031)。

摘　　要：为了评估海带多糖(laminaria japonica polysaccharide,LJP)对辐射诱导的唾液腺功能损伤的影响及其作用机制,对8周龄的雌性昆明小鼠给予^(60)Coγ射线(一次性单次照射,剂量15 Gy,剂量率1250 cGy/min)照射和LJP干预,于照射后28 d进行唾液流量检测和苏木精-伊红染色,并通过免疫组化和RT-qPCR等实验方法来分析下颌下腺组织中与抗氧化应激有关的重要基因——沉默信息调节因子3(Sirtuin3,Sirt3)和锰超氧化物歧化酶(Manganese superoxide dismutase,MnSOD)的表达。实验结果显示,LJP能改善受照射后小鼠的唾液流量和颌下腺结构的损伤,并上调其组织中Sirt3及MnSOD的表达。这表明LJP可能通过激活下颌下腺组织中Sirt3/MnSOD的表达来改善辐射诱导的下颌下腺功能损伤。The aim of this study is to investigate protective effect and the underlying mechanism of Laminaria Japonica Polysaccharide(LJP)on radiation-induced submandibular gland injury in mice.Eight-week-old female mice were randomly divided into four groups,including control group,LJP group,irradiation group,and irradiation plus LJP group.LJP(100 mg/kg/day)was intraperitoneally injected 3 days before irradiation and for consecutive 7 days(once daily).The mice from irradiation alone and irradiation plus LJP groups were exposed to a single dose of 15 Gy by using the ^(60)Co gamma rays.Histopathology and function of the submandibular glands(SMG)were detected on the 28th day post irradiation.The protein and mRNA expression of Sirt3 and MnSOD were respectively examined by real-time qPCR and immunohistochemical staining methods.Results showed that LJP injection significantly increased saliva flow rates in irradiated mice,improved the morphology and structure of SMG,and markedly up-regulated expressions of the Sirt3 and MnSOD.This suggests that LJP treatment could alleviate radiation-induced submandibular gland injury through activation of the Sirt3/MnSOD pathway.

关 键 词：海带多糖 下颌下腺 沉默信息调节因子3 锰超氧化物歧化酶 放射性口干 

分 类 号：R142[医药卫生—公共卫生与预防医学]