二次谐波成像原理及在眼科中的研究进展  

The Principle of Second Harmonic Generation and Its Progress in Ophthalmology Research

作　　者：何欢欢 高蓉蓉 王勤美 黄锦海 He Huanhuan;Gao Rongrong;Wang Qinmei;Huang Jinhai(Eye Hospital,Wenzhou Medical University,Wenzhou 325027,China)

机构地区：[1]温州医科大学附属眼视光医院,325027

出　　处：《中华眼视光学与视觉科学杂志》2021年第6期471-475,共5页Chinese Journal Of Optometry Ophthalmology And Visual Science

基　　金：浙江省医坛新秀卫生高层次人才资助项目(2017-102);温州医科大学附属眼视光医院重大课题(YNZD1201903);温州医科大学附属眼视光医院院内科研启动课题(KYQD20190701)。

摘　　要：二次谐波(SHG)成像利用非线性光学效应,具有空间分辨率高、无需染色、无光毒性等优点,在医学领域已经得到广泛应用。眼部的角膜基质、小梁网、巩膜和神经节细胞轴突中的微管等结构主要呈高度非中心对称,可以采用SHG成像直观地评估。SHG成像在角膜扩张性疾病、角膜感染和青光眼等眼病的诊治方面已有初步应用。现对SHG的产生原理、成像特点及其在眼科的应用进行综述。Second harmonic generation(SHG)imaging is based on an onlinear optical effect.It has been widely studied in the medical field due to its advantages in wide spatial resolution,no need for staining,small photobleaching,and no phototoxicity.The ocular structures,including the corneal stroma,the trabecular meshwork,the sclera and microtubules in the ganglion cell axons are mainly highly non-centrosymmetric structures,which can be visually evaluated by SHG imaging technology.SHG imaging has been used in the diagnosis and treatment of eye diseases such as corneal dilatation,keratoconus,corneal infection and glaucoma.This paper briefly describes the principle,imaging characteristics and applications of SHG in ophthalmology.

关 键 词：二次谐波成像 生物力学 角膜 巩膜 胶原纤维 

分 类 号：R770.4[医药卫生—眼科]