枯草芽孢杆菌对早熟禾和紫花苜蓿修复镉污染土壤的强化作用  

Bioaugmentation of Bacillus subtilis on Remediation of Cadmium-Contaminated Soil by Kentucky Bluegrass(Poa pratensis L.)and Alfalfa(Medicago sativa L.)

作　　者：李琪 邢颖娜 杜逢慧 傅晓文 季蕾 李天元 王加宁 陈贯虹 郭书海 张强[1] LI Qi;XING Ying-na;DU Feng-hui;FU Xiao-wen;JI Lei;LI Tian-yuan;WANG Jia-ning;CHEN Guan-hong;GUO Shu-hai;ZHANG Qiang(Shandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250013, China;Shandong Shanye Environmental Engineering Co., Ltd., Jinan 250101, China)

机构地区：[1]齐鲁工业大学(山东省科学院)生态研究所,山东省应用微生物重点实验室,济南250013 [2]山东山冶环境工程有限公司,济南250101

出　　处：《科学技术与工程》2021年第17期7385-7390,共6页Science Technology and Engineering

基　　金：山东省科学院青年基金(2020QN0014,2020QN0016)。

摘　　要：植物-微生物联合修复是一种经济可行且生态良好的重金属污染土壤修复方法。通过盆栽试验,探究接种枯草芽孢杆菌对两种草坪植物(早熟禾、紫花苜蓿)去除土壤中镉效率的影响及机制。结果表明,种植早熟禾时,枯草芽孢杆菌能够阻隔早熟禾吸收镉,显著提高生物量,增加土壤酶活性,降低土壤有效态镉含量,并且将土壤中总镉的去除率提高约49%;对于紫花苜蓿,枯草芽孢杆菌能够促进紫花苜蓿吸收镉,显著提高生物量,增加土壤酶活性、微生物物种多样性以及有效态镉含量,并且将土壤中总镉去除率提高约140%。可见,接种枯草芽孢杆菌对两种草坪植物修复镉污染土壤均表现出显著的强化作用,而且能够改善只种植紫花苜蓿造成的土壤微生态环境破坏。研究接种枯草芽孢杆菌对两种草坪植物不同的影响和强化机制将为重金属污染场地的植物-微生物修复策略的制订与实施提供理论支持。Plant-microbe combined remediation is an economical,feasible,and environmental-friendly technology in decontamination of soils polluted by heavy metals.Pot experiment was conducted to investigate the effects and mechanisms of Bacillus subtilis inoculation on the removal efficiency of cadmium from soil by Kentucky bluegrass(Poa pratensis L.)and alfalfa(Medicago sativa L.).The results show that in pots with Kentucky bluegrass,the cadmium content of plants and bioavailable cadmium content in soils are decreased,the plant biomass and soil enzyme activity are improved,and the removal efficiency of cadmium is increased by 49%in the presence of Bacillus subtilis.In pots with alfalfa,the plant cadmium content and biomass and soil enzyme activity,microbial diversity,and bioavailable cadmium content are significantly enhanced,and the removal efficiency of cadmium is augmented by 140%in the presence of Bacillus subtilis.These results indicate significant bioaugmentation of Bacillus subtilis inoculation on remediation of cadmium-contaminated soil by two lawn plants.The microecological environment in soil,which is damaged by planting of alfalfa alone,is ameliorated by inoculation.The study on the different effects of Bacillus subtilis inoculation on the two lawn plants and the enhancement mechanism will provide theoretical support for the formulation and implementation of phyto microbial remediation strategies for heavy metal-contaminated sites.

关 键 词：重金属 酶活性 微生物多样性 草坪植物 土壤修复 

分 类 号：X53[环境科学与工程—环境工程]