CK2α在甲状腺肿瘤中的表达及其在癌转移中的作用机制研究  

Study on the expression of CK2αin thyroid tumors and its mechanism of action in cancer metastasis

作　　者：窦娟 李恒宇 陈静 刘宙 Dou Juan;Li Hengyu;Chen Jing;Liu Zhou(Department of Throat and Breast Surgery,the First Affiliated Hospital of Naval Military Medical University,Shanghai 200433,China;Central Plains Clinic,Dental Clinic,Yangpu District,Shanghai 200438,China)

机构地区：[1]海军军医大学第一附属医院甲乳外科,上海200433 [2]上海市杨浦区牙病防治所中原门诊部,上海200438

出　　处：《肿瘤代谢与营养电子杂志》2021年第3期261-266,共6页Electronic Journal of Metabolism and Nutrition of Cancer

基　　金：上海市科研计划项目(18ZR1438600)。

摘　　要：目的探讨蛋白激酶CK2的催化亚基(CK2α)在甲状腺癌中的表达水平,并对CK2α在甲状腺癌中的生物学功能及内在机制进行评价。方法20对甲状腺癌组织和邻近非肿瘤组织样本来自海军军医大学第一附属医院的手术患者,并采用qRT-PCR检测CK2α在甲状腺癌组织和不同人甲状腺癌细胞株中的表达情况;采用CKK-8法检测CK2α对甲状腺癌细胞增殖能力的影响;划痕实验和Transwell法检测CK2α对甲状腺癌细胞迁移和侵袭能力的影响;采用qRT-PCR分析CK2α对YBX1表达调控的关系。结果CK2α在甲状腺癌组织和细胞株中表达明显上调。此外,降低CK2α表达显著抑制了甲状腺癌细胞的增殖、迁移和侵袭,相反过表达CK2α则促进了甲状腺癌细胞的增殖、迁移和侵袭能力。从机制层面上,下调CK2α表达抑制YBX1表达,从而抑制甲状腺癌细胞得生物学功能。结论CK2α通过促进YBX1表达,从而促进甲状腺癌的发生发展。Objective To investigate the expression level of CK2αin thyroid carcinoma,and to evaluate the biological function and its mechanism of CK2αin thyroid carcinoma.Methods Twenty pairs of clinical thyroid carcinoma tissues and corresponding paracancerous normal tissues were obtained,and the expression of CK2αin thyroid carcinoma tissues and different human thyroid cancer cell lines were detected by qRT⁃PCR analysis.The effect of CK2αon cell proliferation of thyroid cancer cells was detected by CKK⁃8 method.The effects of CK2αon the migration and invasion of thyroid cancer cells were detected by wound healling test and Transwell test.The qRT⁃PCR was used to analyze the relationship between CK2αand YBX1 expression.Results CK2αwas significantly up⁃regulated in thyroid carcinoma tissues and cell lines.In addition,decreased CK2αexpression significantly inhibited the proliferation,migration and invasion of thyroid cancer cells,whereas overexpression of CK2αpromoted the proliferation,migration and invasion of thyroid cancer cells.Mechanistically,we found that down⁃regulation of CK2αexpression inhibited YBX1 expression,thereby inhibiting the biological function of thyroid cancer cells.Conclusion CK2αpromotes the development of thyroid cancer by promoting the expression of Y-box binding protein 1.

关 键 词：蛋白激酶CK2的催化亚基 甲状腺肿瘤 迁移和侵袭 

分 类 号：R736.1[医药卫生—肿瘤]