围手术期使用抗生素对伴高脂血症或糖尿病的牙周炎大鼠颈动脉壁及血清白介素-6影响的研究  

Study on the effect of antibiotics application in perioperative period on carotid artery and serum interleukin-6 in periodontitis rats with hyperlipidemia or diabetes

作　　者：王冲 李豪 赵勇 程瑞 石学雪[1] 高晋华 任秀云[1] Wang Chong;Li Hao;Zhao Yong;Cheng Rui;Shi Xuexue;Gao Jinhua;Ren Xiuyun(Department of Periodontology,Shanxi Medical University School and Hospital of Stomatology&Shanxi Province Key Laboratory of Oral Diseases Prevention and New Materials,Taiyuan 030001,China)

机构地区：[1]山西医科大学口腔医学院·口腔医院牙周科口腔疾病防治与新材料山西省重点实验室,太原030001

出　　处：《中华口腔医学杂志》2021年第6期557-564,共8页Chinese Journal of Stomatology

基　　金：国家自然科学基金(81271144);中央引导地方科技发展资金项目(YDZX20201400001131);山西省高等学校科学研究优秀成果培育项目(2020KJ014)。

摘　　要：目的探讨围手术期使用抗生素对伴高脂血症(hyperlipidemia,HL)或糖尿病(diabetes mellitus,DM)慢性牙周炎(chronic periodontitis,CP)大鼠颈动脉血管及血清白介素-6(interlenkim-6,IL-6)水平的影响。方法分两批次建立HL+CP及DM+CP大鼠模型。分组如下,A和A′组:均为正常对照组,每组各7只;B(HL)和B′(DM)组:每组各7只;C(HL+CP)和C′(DM+CP)组,每组各21只。模型建成后将C和C′组分别分为C1和C1′组(均为自然进程组),C2和C2′组(均为单纯拔牙组),C3和C3′组(均为拔牙+抗生素组),每组各7只。C2、C2′、C3、C3′组分2次分别拔除左右实验牙(双侧上颌第一、二磨牙)。拔牙前后分5个时间点(T1~T5)采集血清(第1次拔牙前为T1,第1次拔牙后1周为T2,第2次拔牙后1、3、5周分别为T3、T4和T5),酶联免疫吸附测定(enzyme-linked immunosorbent assay,ELISA)法检测血清IL-6的绝对含量,实验组IL-6绝对含量与同批次对照组绝对含量的比值即为IL-6的相对含量。实验结束后处死所有大鼠,取颈动脉分叉血管组织,观察血管病理及斑块形成情况,并测量颈动脉内中膜层厚度(intimal-medial membrane,IMT)。结果大鼠颈动脉光镜下病理结果显示,C组大鼠IMT明显增厚[C1、C2、C3组IMT分别为(125.1±21.6)、(142.2±21.0)及(93.9±14.4)μm],均显著大于A组[(61.0±10.4)μm](P<0.01),其中C2组增厚最明显。C1、C2组弹性纤维排列紊乱甚至断裂、消失,出现典型的动脉粥样硬化斑块,斑块表现为弥漫性钙盐沉积,存在于内中膜层并向管腔突起。C3组弹性纤维排列相对整齐,未见明显断裂现象,斑块数量明显减少。B′、C′组大鼠IMT未见明显增厚,血管壁不完整,弹性纤维排列紊乱、断裂,平滑肌细胞空泡性变,C2′组可见血管壁明显变薄,出现动脉钙化斑块,表现为多发性钙化灶,贯穿内中膜甚至全层。C3′组血管组织完整性较好,弹性纤维排列相对整齐,斑块数量减少。随着时间延长,IL-6相�Objective To investigate the effect of antibiotics application in perioperative period on carotid artery and serum interluekin-6(IL-6)in chronic periodontitis(CP)rats with hyperlipidemia(HL)or diabetes mellitus(DM).Methods The models of CP rats with HL or DM were established in different batches.The rats were divided into groups as follows:A and A'were normal control groups,n=7 in each group;B(HL)and B'(DM)were groups of HL and DM,n=7 in each;C(HL+CP)and C'(DM+CP)were groups of CP with HL and CP with DM,n=21 in each.After the establishment of the models,groups C and C'were divided into C1 and C1'non-intervention group,C2 and C2'simple tooth extraction group and C3 and C3'antibiotic-assisted tooth extraction,with 7 rats in each of the groups.Two time interventions were performed to extract experimental teeth(bilateral maxillary first and second molars)in groups C2,C2',C3 and C3'.Serum samples were collected at 5 time points before and after tooth extraction(T1:before the first tooth extraction;T2:one week after the first tooth extraction;T3,T4,T5:the first,third and fifth week after the second tooth extraction),respectively.The absolute content of IL-6 in serum was detected by enzyme-linked immunosorbent assay(ELISA).Ratio of the absolute content in the experimental groups and control groups were calculated as the relative content of IL-6.At the end of the experiments,all rats were euthanasia and the bifurcation vascular tissues of carotid artery were collected for the observation of the carotid artery pathology and plaque formation and the measurement of the thickness of carotid intimal-medial layer(IMT).Results Carotid artery pathology observation showed the IMT in group C was significantly thickened and the group C2 was the most thickened one(compared with group A,P<0.01).Elastic fibers in groups C1 and C2 were disordered or even broken and disappeared,presenting typical atherosclerotic plaques,which were diffuse calcium salt deposits in the intimal-medial layers and protrude into the lumen.Elastic fibers in gr

关 键 词：慢性牙周炎 高脂血症 糖尿病 动脉粥样硬化 颈动脉血管 

分 类 号：R781.42[医药卫生—口腔医学] R587.1[医药卫生—临床医学] R589.2