miRNA-1290在胃癌患者血清中的相对表达水平及临床意义  

The relative level and clinical significance of miRNA-1290 in theserum of patients with gastric cancer

作　　者：刘倩[1] 段莎 LIU Qian;DUAN Sha(No.2 Department of Gastroenterology,Shaanxi Provincial People′s Hospital,Xi′an,Shanxi 710068,China)

机构地区：[1]陕西省人民医院消化二科,陕西西安710068

出　　处：《检验医学与临床》2021年第13期1911-1914,共4页Laboratory Medicine and Clinic

摘　　要：目的探究微小RNA-1290(miRNA-1290)在胃癌患者血清中的相对表达水平及临床意义。方法选取2018年3月至2020年9月该院消化内科收治的64例胃癌患者为胃癌组,另选取同时间段在该院进行体检的64例健康者作为对照组,采集所有入组人员血液标本,采用实时荧光定量聚合酶链反应检测血清miRNA-1290相对表达水平,分析血清miRNA-1290相对表达水平与患者临床特征的关系,绘制受试者工作特征曲线评估血清miRNA-129对胃癌的诊断价值。结果胃癌组血清miRNA-1290相对表达水平明显高于对照组,差异有统计学意义(t=42.393,P<0.001);不同肿瘤分期(TNM分期)、淋巴结转移情况、分化程度、肿瘤直径胃癌患者血清miRNA-1290相对表达水平差异有统计学意义(P<0.05),不同性别、年龄、病理类型胃癌患者血清miRNA-1290相对表达水平差异无统计学意义(P>0.05);miRNA-1290诊断胃癌的灵敏度为91.4%,特异度为87.3%,曲线下面积为0.816(95%CI:0.691~0.942,P<0.05),最佳截断值为4.92。结论miRNA-1290在胃癌患者血清中相对表达水平升高,对胃癌具有一定的诊断价值,可作为胃癌早期诊断的一项重要分子标志。Objective To explore the expression and clinical significance of miRNA-1290 in the serum of patients with gastric cancer.Methods A total of 64 patients with gastric cancer admitted to the Department of Gastroenterology of the hospital from March 2018 to September 2020 were selected as the gastric cancer group,and 64 healthy patients who underwent physical examinations in the hospital during the same time period were selected as the control group.Blood samples of all participants were collected,and real-time fluorescent quantitative polymerase chain reaction(qRT-PCR)was used to detect serum miRNA-1290 levels.The relationship between serum miRNA-1290 expression and the patient′s clinical characteristics was analyzed,and the receiver operating characteristic curve was drawn to evaluate the diagnostic value of serum miRNA-129 for gastric cancer.Results The expression level of serum miRNA-1290 in the gastric cancer group was significantly higher than that in the control group,and the difference was statistically significant(t=42.393,P<0.001).There were significant differences in serum miRNA-1290 expression levels in patients with gastric cancer with different TNM stages,lymph node metastasis,degree of differentiation,and tumor diameter(P<0.05).There were no significant differences in serum miRNA-1290 expression levels in patients with gastric cancer of different genders,ages,and pathological types(P>0.05).The sensitivity of miRNA-1290 in diagnosing gastric cancer was 91.4%,specificity was 87.3%,area under curve was 0.816(95%CI:0.691-0.942,P<0.05)and the cutoff value was 4.92.Conclusion The expression level of miRNA-1290 in the serum of gastric cancer patients is elevated,which has certain diagnostic value for gastric cancer and can be used as an important marker molecule for early diagnosis of gastric cancer.

关 键 词：微小RNA-1290 胃癌 诊断 

分 类 号：R735.2[医药卫生—肿瘤]