肺浸润性腺癌组织中miR-153-3p和PRDM2的表达  

Expression and Correlation of MiR-153-3p and PRDM2 in Lung Invasive Adenocarcinoma

作　　者：曲靓靓 朱丽娟 杨薇 Qu Liangliang;Zhu Liping;Yang Wei(The first Affiliated Hospital of Jinzhou Medical University,Jinzhou 121000 China)

机构地区：[1]锦州医科大学附属第一医院,辽宁锦州121000

出　　处：《锦州医科大学学报》2021年第3期99-102,共4页Journal of Jinzhou Medical University

基　　金：辽宁省科学技术计划项目,项目编号:201602304。

摘　　要：目的观察肺浸润性腺癌组织中微小RNA-153-3p(microRNA-153-3p,miR-153-3p)和正性调控域锌指蛋白(positive regulatory domain zinc finger protein,PRDM2)的表达,分析其关联性及临床意义。方法选择56例肺浸润性腺癌患者作为研究对象,留取术后肿瘤组织作为观察组,留取正常肺组织作为作为对照组。应用实时荧光定量PCR(qRT-PCR)检测两组中miR-153-3p的表达,应用免疫组化法检测肺浸润性腺癌组织中PRDM2的表达。结果肺浸润性腺癌中miR-153-3p的表达明显低于对照组(P<0.05),MiR-153-3p在不同肿瘤最大径、肺膜累犯分组的表达中差别有统计学意义(P<0.05)。MiR-153-3p的表达与预后有关(P<0.05)。肺浸润性腺癌中miR-153-3p与PRDM2具有负相关性(r=-0.69,P=0.013)。结论MiR-153-3p在肺浸润性腺癌中的表达下降,其与病变的形成和进展有关。MiR-153-3p与PRDM2可能具有协同负向作用。Objective To observe the expression of microRNA-153-3p(miR-153-3p)and positive regulatory domain zinc finger protein2(PRDM2)in lung invasive adenocarcinoma,and analyze their correlation and clinical significance.Methods A total of 56 cases of lung invasive adenocarcinoma were selected as the research object,neoplasm tissue were selected as the observation group,and normal lung tissue were seletcted as the control group.The expression of miR-153-3p was detected by real-time fluorescent quantitative PCR(qRT-PCR)in two groups.PRDM2 was detected by immunohistochemistry method in observation group.Results Expression of miR-153-3p was significantly lower in lung invasive adenocarcinoma than that in the control group(P<0.05).Expression of miR-153-3p was significantly different in maximum diameter and pleura recidivism(P<0.05).Expression of miR-153-3p is related to prognosis(P<0.05).Negative correlation was found between miR-153-3p and PRDM2 in lung invasive adenocarcinoma(r=-0.69,P=0.013).Conclusion Expression of miR-153-3p decreased in lung invasive adenocarcinoma,which is related to the formation and progression.MiR-153-3p and PRDM2 may have negative synergistic effects.

关 键 词：肺浸润性腺癌 miR-153-3p PRDM2 预后 

分 类 号：R734.2[医药卫生—肿瘤]