艾迪注射液对N-亚硝基二乙胺(DEN)诱导的大鼠肝癌模型的干预作用  

Intervention effect of Aidi injection on rat liver cancer model induced by N-nitrosodiethylamine(DEN)

作　　者：韩会靖 施兴海 孙丽华[3] HAN Huijing;SHI Xinghai;SUN Lihua(Institute of Drug Inspection,Xinjiang Uygur Autonomous Region,Urumqi 830011;Department of Clinical Laboratory,First People's Hospital,Urumqi 830002;Department of Liver Center,the First Affiliated Hospital of Xinjiang Medical University,Urumqi 830054,China)

机构地区：[1]新疆维吾尔自治区药品检验研究院,乌鲁木齐830011 [2]新疆维吾尔自治区乌鲁木齐市第一人民医院实验室,乌鲁木齐830002 [3]新疆医科大学第一附属医院肝病中心,乌鲁木齐830054

出　　处：《新疆医科大学学报》2021年第7期782-787,共6页Journal of Xinjiang Medical University

基　　金：国家自然科学基金(82060121)。

摘　　要：目的探讨艾迪注射液对N-亚硝基二乙胺(diethylnitrosamine,DEN)诱导的Wistar大鼠肝癌模型的干预作用。方法健康Wistar大鼠30只,随机分为对照组、模型组、艾迪注射液处理组,每组10只。对照组按0.1 mL/kg的剂量腹腔注射生理盐水,每周注射1次,共注射20 w。模型组大鼠给予一次性腹腔注射100 mg/kg体重的DEN,后给予0.05%DEN自由饮水。艾迪注射液处理组大鼠开始腹腔注射DEN时即给予艾迪注射液1 mL/kg,后每周注射1次,共注射20周,取血测定大鼠血清中天门冬氨酸转移酶(aspartate aminotransferase,AST)、丙氨酸转移酶(alanine aminotransferase,ALT)、碱性磷酸酶(alkaline phosphatase,ALP)含量,取各组大鼠肝脏组织进行病理学检测。用免疫组织化学染色检测肝组织中α-平滑肌肌动蛋白(α-smooth muscle actin,α-SMA)和CD34蛋白的表达。结果(1)与对照组比较,模型组大鼠血清中ALT、AST、ALP均明显增高,差异有统计学意义(P<0.05);与模型组比较,艾迪注射液处理组ALT、ALP明显降低,差异有统计学意义(P<0.05),AST差异无统计学意义。(2)病理结果显示:对照组肝组织结构及细胞形态正常;模型组肝组织结构破坏,肝细胞损伤严重,大量胶原纤维将癌变的肝细胞分隔呈灶状分布,Masson染色显示大量蓝色胶原纤维增生,包裹坏死区;艾迪注射液处理组可见假小叶增多,Masson染色可见大量胶原纤维分隔形成的假小叶,有大量炎细胞浸润,可见干酪样坏死。(3)与对照组比较,模型组、艾迪注射液处理组肝癌组织中α-SMA、CD34表达升高,差异有统计学意义(P<0.01);与模型组比较,艾迪注射液处理组的α-SMA、CD34明显降低,差异有统计学意义(P<0.01)。结论艾迪注射液对DEN所致肝癌大鼠有一定的疗效,艾迪注射液能缓解肝癌所致的肝组织损伤,艾迪注射液干预的大鼠α-SMA和CD34表达有降低趋势,艾迪注射液可能通过抑制α-SMA和CD34表达,抑制肿瘤的浸润转移,�Objective To investigate the intervention effect of Aidi injection on the Wistar rat liver cancer model induced by N-nitrosodiethylamine(DEN).Methods Thirty Wistar rats were randomly and equally divided into control group,model group,and Aidi injection treatment group.The control group was injected intraperitoneally with normal saline at a dose of 0.1 mL/kg,once a week,for a total of 20 weeks.Rats in the model group were given a one-time intraperitoneal injection of 100 mg/kg of DEN,followed by 0.05%DEN with free drinking water.Rats in the Aidi injection treatment group were given Aidi injection 0.5 mL/kg when they started intraperitoneal injection of DEN,and then injected once a week for a total of 20 weeks.The serum aspartate transferase(AST),alanine transferase(ALT),alkaline phosphatase(ALP)was tested,the pathological examination of liver tissues were tested.Immunohistochemical staining was used to detect the expression of alpha smooth muscle actin(α-SMA)and CD34 protein in liver tissue.Results⑴Compared with the control group,the serum ALT,AST,and ALP in the model group were significantly increased,and the differences were statistically significant(P<0.05).Compared with the model group,the ALT and ALP of the Aidi injection treatment group were significantly reduced,and the difference was statistically significant(P<0.05).However,there was no difference on AST between model and Aidi group.⑵The control group were normal in liver structure and cell morphology.The model group was destroyed in liver,the liver cells were seriously damaged,and a large number of collagen fibers separated the cancerous liver cells into focal distribution.Compared with the control group,the Aidi injection treatment group showed there were more pseudolobules,a large number of inflammatory cell infiltration,and caseous necrosis.⑶Compared with the control group,the expression ofα-SMAand CD34 in both model and Aidi group was higher,the difference was statistically significant(P<0.01).Compared with the model group,the expression ofα

关 键 词：艾迪注射液 N-亚硝基二乙胺 肝癌 Α-平滑肌肌动蛋白 

分 类 号：R365[医药卫生—病理学]