基于HPLC-ESI-MS技术的桑白皮、桑根皮及外粗皮主要成分定性与定量研究  

Systematic analysis on chemical constituents of Mori Cortex, mulberry root bark and its phellem layer based on HPLC-ESI-MS

作　　者：郑甜碧 万晶琼 杨翠云[1] 魏渊[1] 闻崇炜[1] 欧阳臻[1] ZHENG Tian-bi;WAN Jing-qiong;YANG Cui-yun;WEI Yuan;WEN Chong-wei;OUYANG Zhen(School of Pharmacy,Jiangsu University,Zhenjiang 212013,China;School of Food and Biological Engineering,Jiangsu University,Zhenjiang 212013,China)

机构地区：[1]江苏大学药学院,江苏镇江212013 [2]江苏大学食品与生物工程学院,江苏镇江212013

出　　处：《中国中药杂志》2021年第9期2237-2244,共8页China Journal of Chinese Materia Medica

基　　金：中央本级重大增减支项目(2060302);国家自然科学基金项目(81573529)。

摘　　要：该研究建立高效液相-电喷雾质谱联用(HPLC-ESI-MS)及HPLC从定性定量两方面探讨桑白皮及未去外粗皮的桑根皮主要化学成分及含量的差异。定性采用HPLC-ESI-MS,在正、负离子模式下进行检测,鉴定出桑根皮33种化合物,桑白皮22种化合物,外粗皮26种化合物。桑根皮与桑白皮共有成分22种,桑根皮特有成分11种,桑白皮与外粗皮共有成分15种,桑白皮特有成分7种。定量采用HPLC,分析桑根皮与桑白皮中7种主要成分桑皮苷A、绿原酸、二氢桑色素、氧化白藜芦醇、桑辛素O、桑酮G和桑酮H的含量,在考察的浓度范围内测定的成分线性关系良好(r>0.998 9),加样回收率为99.88%~103.0%,RSD为1.7%~2.9%;13批桑根皮、桑白皮样品中7种成分含量存在差异,去皮加工后,桑皮苷A、绿原酸、二氢桑色素及桑辛素O含量均较未去皮前升高,而氧化白藜芦醇、桑酮G及桑酮H含量较未去皮前均降低。实验结果可为桑白皮要求去除外粗皮的合理性及质量控制提供依据。In this study, HPLC-ESI-MS and HPLC methods were established to explore the differences in the main chemical components and content of Mori Cortex with(mulberry root bark) and without(Mori Cortex) the phellem layer from both qualitative and quantitative aspects. The HPLC-ESI-MS method was used for quality analysis in positive and negative ion modes, and 33 compounds were identified in mulberry root bark, 22 compounds in Mori Cortex, and 26 compounds in phellem layer;mulberry root bark and Mori Cortex shared 22 components, and mulberry root bark has 11 unique compounds;Mori Cortex and its phellem layer shared 15 components, while Mori Cortex has 7 unique compounds. HPLC method was used to simultaneously determine 7 major constituents, including mulberroside A, chlorogenic acid, dihydromorin, oxyresveratrol, moracin O, kuwanon G, and kuwanon H, and the developed method showed good linearity(r>0.998 9) within the concentration range and the recoveries varied from 99.88% to 103.0%, and the RSD was 1.7%-2.9%. The HPLC results showed that the contents of the 7 compounds have great differences in 13 batches samples, compared with mulberry root bark, the contents of mulberroside A, chlorogenic acid, dihydromorin and moracin O of Mori Cortex were increased, while the contents of oxyresveratrol, kuwanon G and kuwanon H were decreased after peeling process. These results can provide a basis for the rationality and quality control of Mori Cortex required to remove the phellem layer.

关 键 词：桑白皮 HPLC-ESI-MS 去皮加工 化学成分 定性定量 

分 类 号：R284.1[医药卫生—中药学]