构建兔颈总动脉粥样硬化斑块模型的新方法及评价  

Establishment and evaluation of a rabbit model of carotid atherosclerotic plaque

作　　者：叶鹏飞 郭应坤[1] 张怡[2] 沈梦婷 李湘 陈林 徐婷 文凌仪 YE Pengfei;GUO Yingkun;ZHANG Yi;SHEN Mengting;LI Xiang;CHEN Lin;XU Ting;WEN Lingyi(Department of Radiology,West China Second Hospital,Sichuan University,Key Laboratory of Birth Defects and Related Diseases of Women and Children,Ministry of Education,Chengdu 610041,China;Department of Radiology,West China Hospital,Sichuan University,Chengdu 610041)

机构地区：[1]四川大学华西第二医院放射科,出生缺陷与相关妇儿疾病教育部重点实验室,成都610041 [2]四川大学华西医院放射科,成都610041

出　　处：《中国实验动物学报》2021年第3期301-306,共6页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家自然科学基金(81971586);四川省应用基础研究项目(2019YJ0087)。

摘　　要：目的探索经兔耳中央动脉穿刺入路,利用球囊损伤兔颈总动脉内膜联合高脂饲料喂养,构建兔颈总动脉粥样硬化模型的新方法并评价可行性。方法将兔经高脂饲料预喂养4周后,经兔耳中央动脉穿刺入路,利用球囊损伤右侧兔颈总动脉内膜,再经高脂饲料喂养8周。利用磁共振仪和病理组织学观察兔颈总动脉病变。结果本研究构建兔颈总动脉粥样硬化动物模型的成功率为85%。在磁共振图像上,实验组颈总动脉血管壁增厚,明显强化,且实验组动脉粥样硬化斑块的信号强度和信噪比明显高于自身对照组。实验组颈总动脉血管壁内膜呈现动脉粥样化斑块的病理表现,而自身对照兔颈总动脉血管壁内膜正常。结论通过兔耳中央动脉穿刺入路,行球囊损伤兔颈总动脉内膜联合高脂饲料喂养的方法成功构建了兔颈总动脉粥样硬化斑块模型,该方法具有创伤小、操作简单、重复性较强、造模用时短以及成模率高等优点。球囊损伤颈总动脉内膜采用了独创的兔耳中央动脉穿刺入路,为构建兔颈总动脉粥样硬化斑块模型开辟了新思路。Objective To establish a rabbit model of carotid atherosclerotic plaque by puncturing from central artery of rabbit ear and high-fat diet.Methods All rabbit were fed with high-fat diet for 4 weeks.By puncturing from central artery of rabbit ear,the balloon was inflated to injury intima of carotied artery and then followed with 8 weeks of high-fat diet.The right common carotid artery which damaged by balloon catheter were defined as experimental group and the left common carotid artery as self-control group.MRI and histopathology were used to evaluate common cervical artery lesions in rabbits.Results The success rate of modeling was 85.0%.On MRI scan image,the wall of carotid artery in the experimental group was thickened and obviously strengthened,while there was no thickening of carotid artery wall in the control group,and no obvious enhancement was observed.In the experimental group,the intima of the common carotid artery wall presented the pathological manifestations of atherosclerotic plaques.In the control group,the inner membrane of carotid artery wall was normal.Conclusions A rabbit model of carotid atherosclerosis was successfully constructed with balloon injury by puncturing from central artery of rabbit ear accompanied high-fat diet.The modified method have advantages of small trauma,simple operation,strong repeatability,short modeling time and high success rate.

关 键 词：动脉粥样硬化 动物模型 球囊损伤 高脂饮食 兔 颈总动脉 

分 类 号：Q95-33[生物学—动物学]