利用InDel标记进行啤酒大麦黄花叶病抗性鉴定  

Identification of Malting Barley Germplasm with Resistance to Barley Yellow Mosaic Disease by Using InDel Markers

作　　者：徐肖 栾海业[1] 杨红燕 黄煜韬 张英虎[1] 臧慧[1] 陶红[1] 陈和[1] 陈健[1] 乔海龙[1] 沈会权[1] XU Xiao;LUAN Hai-ye;YANG Hong-yan;HUANG Yu-tao;ZHANG Ying-hu;ZANG Hui;TAO Hong;CHEN He;CHEN Jian;QIAO Hai-long;SHEN Hui-quan(Jiangsu Coastal Area Institute of Agricultural Sciences,Yancheng 224001,China;Jiangsu Tianfu Biological Technology Co.,Ltd.Yancheng 224001,China)

机构地区：[1]江苏沿海地区农业科学研究所,江苏盐城224001 [2]江苏天赋生物科技有限责任公司,江苏盐城224001

出　　处：《大麦与谷类科学》2021年第3期53-57,共5页Barley and Cereal Sciences

基　　金：国家大麦青稞产业技术体系(CARS-05);江苏省农业科技自主创新项目[CX(19)3062];2018年江苏省苏北科技专项(SZ-YC2018013);江苏省现代农业产业技术体系(JATS[2020]207)。

摘　　要：大麦黄花叶病是严重威胁冬大麦生产的重大病害之一。为促进抗黄花叶病基因在大麦育种中的应用,利用插入或缺失(inserticon-deletion,InDel)标记JSB056和JSB060对180份啤酒大麦资源进行基因型检测,结合病圃黄花叶病表型鉴定,并评价标记在育种中应用的有效性。结果表明,聚合2个InDel标记具有较好的检测效果,检测准确率高达91.9%,是分子辅助选择育种中鉴定大麦黄花叶病抗性的较理想标记。Barley yellow mosaic disease is one of the major diseases that seriously threaten the production of winter barley.In order to promote the application of the genes for resistance to yellow mosaic disease in barley breeding,we used the InDel markers JSB056 and JSB060 to determine the genotypes of 180 accessions of malting barley germplasm,combined with identification of the phenotypes of yellow mosaic disease in diseased plant nursery,and to evaluate the effectiveness of the markers in breeding.The results showed that the combinative use of the two InDel markers is highly effective in identifying resistance genes,with the identification accuracy rate of 91.9%.Therefore,they are appropriate markers for identification of barley germplasm with resistance to barley yellow mosaic disease,so they are useful tools in molecular-assisted selection breeding.

关 键 词：啤酒大麦 大麦黄花叶病 INDEL标记 抗性鉴定 

分 类 号：S512.1[农业科学—作物学] S311