火炭母水提液体内外抗氧化活性研究  

Study on Antioxidant Activity of Polygonum chinese L.Water Extracts in vitro and in vivo

作　　者：杨艾华 宋姗姗 林宇杰 胡小娇 王秋 王微微 YANG Ai-hua;SONG Shan-shan;LIN Yu-jie(College of Basic Medical Sciences,Zunyi Medical University,Zunyi,Guizhou 563000)

机构地区：[1]遵义医科大学基础医学院,贵州遵义563000

出　　处：《安徽农业科学》2021年第13期184-186,193,共4页Journal of Anhui Agricultural Sciences

基　　金：贵州省科技计划项目(黔科平台人才〔2019〕-021);遵义医科大学大学生创新创业训练计划(ZYDC2019041)。

摘　　要：[目的]研究火炭母水提液的体内及体外抗氧化活性。[方法]采用体外试验测定火炭母水提液对超氧阴离子自由基和羟基自由基的清除能力;体内试验以秀丽隐杆线虫为模型,测定火炭母水提液对线虫寿命和应激损伤的影响。[结果]火炭母水提液对超氧阴离子自由基和羟基自由基具有一定的清除能力,并且随着浓度的增加,自由基清除率逐渐增强;50 mg/mL火炭母水提液可以显著延长线虫寿命,并且提高线虫在急性热应激和氧化应激条件下的存活率。[结论]火炭母水提液具有良好的抗氧化活性,该研究为火炭母开发成为天然抗氧化剂和保健产品提供理论基础。[Objective]To study the antioxidant activity of Polygonum chinese L.water extracts in vitro and in vivo.[Method]The in vitro test was used to determine the scavenging ability of the Polygonum chinese water extract on superoxide anion free radicals and hydroxyl free radicals.In vivo,Caenorhabditis elegans were used to study the effects of Polygonum chinese water extracts on lifespan,hot stress and oxidative stress.[Result]The water extract of Polygonum chinese had a certain scavenging ability to superoxide anion free radicals and hydroxyl free radicals,and as the concentration increases,the free radical scavenging rate gradually increased.The treatment with 50 mg/mL Polygonum chinese water extracts could increase the lifespan,and obviously improve the survival rate of C.elegans under hot stress and oxidative stress.[Conclusion]The water extract of Polygonum chinese has good antioxidant activity.This study provides a theoretical basis for the development of Polygonum chinese as a natural antioxidant and health care product.

关 键 词：火炭母 水提液 体内外 抗氧化活性 秀丽隐杆线虫 

分 类 号：R284.1[医药卫生—中药学]