神曲消食口服液对功能性消化不良小鼠胃肠运动的影响及机制  

Effects and mechanism of Shenqu Xiaoshi oral liquid on gastrointestinal motility in mice with functional dyspepsia

作　　者：张丛敏[1] 朱晓静[1] 魏金铠 李红亚[1] 李彩霞 唐卉[1] 靳晨晨[1] 赵永才 ZHANG Congmin;ZHU Xiaojing;WEI Jinkai;LI Hongya;LI Caixia;TANG Hui;JIN Chenchen;ZHAO Yongcai(Department of Pediatrics,First Center Hospital of Baoding,Baoding 071000)

机构地区：[1]保定市第一中心医院儿科,河北保定071000

出　　处：《中国比较医学杂志》2021年第6期83-88,共6页Chinese Journal of Comparative Medicine

基　　金：2018年保定市科学技术研究与发展指导计划(18ZF003)。

摘　　要：目的探究神曲消食口服液对功能性消化不良(FD)小鼠胃肠运动的影响及机制。方法取40只KM小鼠随机分为正常组、神曲消食口服液低、中及高剂量组,检测小鼠血常规和肝肾功能指标。另取50只KM小鼠中随机选出10只作为正常组,剩余小鼠采用不规律进食+左旋精氨酸(L-Arg)法构建FD动物模型,再随机分为模型组、神曲消食口服液低、中及高剂量组;记录小鼠体重情况,计算小鼠胃内残留率和肠推进率,观察胃组织病理学改变,采用实时荧光定量PCR(qRT-PCR)和蛋白印迹(Western blot)检测小鼠胃组织中内质网应激因子肌醇需求酶1(IREl)、肿瘤坏死因子受体相关因子2(TRAF2)的表达。结果各组小鼠白细胞(WBC)、红细胞(RBC)、血红蛋白(HGB)、血小板(PLT)、淋巴细胞(LYM)、单核细胞(MONO)、中性粒细胞(NEU)、天门冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)、总胆汁酸(TBA)、尿素氮(BUN)及肌酐(CRE)比较,组间差异无统计学意义(P>0.05);各组小鼠胃组织粘膜层、粘膜下层、肌层及浆膜层结构清晰,均未见明显的病理学改变;与模型组相比,神曲消食口服液低、中及高剂量组胃排空率和小肠推进率明显升高,胃组织中IREl和TRAF2 mRNA和蛋白的表达明显下降(P<0.05)。结论神曲消食口服液对正常小鼠血常规及肝肾功能无明显影响,可明显改善FD小鼠胃肠运动功能,可能与下调内质网因子IREl和TRAF2的表达有关。Objective To explore the effects and mechanism of Shenqu Xiaoshi oral liquid(SXOL)on gastrointestinal motility in mice with functional dyspepsia(FD).Methods This study involved 50 KM mice.Ten mice were randomly assigned to the normal group,and the remaining 40 mice were used to establish animal models of FD by the irregular eating and L-arginine method.They were then randomly divided into a model group,low-dose SXOL group,medium-dose SXOL group,and high-dose SXOL group.Routine blood and liver/kidney function indexes were measured.Their body weight was recorded,gastric residual rate and intestinal propulsion rate were calculated,and pathological changes in gastric tissues were observed.Real-time quantitative polymerase chain reaction and Western blot were used to detect expression of inositol-requiring enzyme 1(IREl)and tumor necrosis factor receptor-associated factor 2(TRAF2)in gastric tissues.Results There were no significant differences in the white blood cell count,red blood cell count,hemoglobin concentration,platelet count,lymphocyte count,monocyte count,neutrophil count,aspartate aminotransferase concentration,alanine aminotransferase concentration,total bile acid concentration,blood urea nitrogen concentration,or creatinine concentration among all groups(P>0.05).The structure of the gastric mucosal layer,submucosal layer,muscle layer,and serosal layer in each group was clear,and there were no obvious pathological changes.Compared with the model group,the gastric emptying rate and small intestinal propulsion rate were significantly higher in the low-dose,medium-dose,and high-dose SXOL groups,while expression of IREl and TRAF2 mRNA and protein was significantly decreased in gastric tissues(P<0.05).Conclusions SXOL has no significant effects on routine blood and liver/kidney function indices in normal mice.However,SXOL can significantly improve gastrointestinal motility function in mice with FD,which may be related to down-regulation of the expression of the endoplasmic reticulum factors IR11 and TRAF2.

关 键 词：神曲消食口服液 血常规 肝肾功能 功能性消化不良 胃肠运动 

分 类 号：R-33[医药卫生]