miR-222对甲状腺乳头状癌侵袭与转移的作用机制  

The mechanisms of miR-222 promoting invasion and miff ration of papillary'thyroid carcinoma

作　　者：张伟兰 毛玉山[1,2] Zhang Huilan

机构地区：[1]宁波大学医学院附属医院,315020 [2]宁波大学医学院,315211

出　　处：《浙江临床医学》2021年第6期779-781,785,共4页Zhejiang Clinical Medical Journal

基　　金：浙江省医药卫生科技计划项目(2018ZH029);宁波市自然科学基金资助项目(2018A610248);宁波市“科技创新2025”重大专项资助项目(20I9B10035);宁波市社会发展计划资助项目(2019C50080)。

摘　　要：目的探讨miR-222对甲状腺乳头状癌(PTC)侵袭与转移的作用机制。方法培养人PTC癌细胞株BCPAP和K1,构建miR-222稳转株。通过细胞划痕和Transwell实验评估miR-222在PTC细胞迁移和侵袭中的作用。生物信息学分析miR-222的潜在靶标,应用qPCR检测miR-222对ASPP2表达的影响。构建ASPP2双荧光素酶V-UTR报告基因系统确定miR-222与ASPP2结合区域。转染ASPP2表达质粒,确认miR-222对细胞迁移和侵袭影响。经裸鼠尾静脉注射高表达miR-222的BCPAP细胞,评估miR-222在体内转移中的作用。结果miR-222在体外可增强PTC细胞的迁移和侵袭能力,在体内促进肺部转移灶形成。miR-222能抑制ASPP2的表达,其结合位点为ASPP2的3'-UTR区域。结论miR-222通过下调ASPP2表达促进PTC侵袭和转移。Objective To explore the role of miR-222 in the migration and invasion of papillary thyroid carcinoma(PTC).Methods Human PTC cells line BCPAP and KI were cultured and transfected with miR-222.The role of miR-222 in the invasion and migration ofPTC cells was evaluated by cell scratches and transwell assay.Bioinfonnatics was used to analyze the potential targets of miR-222,and qPCR was used to detect the effects of miR-222 on ASPP2 expression.The ASPP2 dual luciferase 3'-UTR.reporter gene system was constructed to confirm the binding region of miR-222 to ASPP2.The effect of miR-222 on cell migration and invasion was confirmed after transfection with ASPP2 expression plasmid.Nude mice were iiijected with BCPAP ceDs over-expressing miR-222 via the tail vein,which was used to evaluate the role of miR-222 on PTC metastasis in vivo.Results miR-222 can enhance the invasion and migration ability ofPTC cells in vitro,and can promote the formation of lung metastasic nodules in vivo.miR-222 can inhibit the expression of ASPP2,and the 3'-UTR region of ASPP2 is the binding site for miR-222.Conclusion miR-222 promotes PTC invasion and metastasis by downregularing the ASPP2.

关 键 词：甲状腺乳头状癌 MIR-222 ASPP2 侵袭 转移 

分 类 号：R73[医药卫生—肿瘤]