玉米SPXs家族基因的鉴定与表达规律分析  

Identification and expression analysis of SPXs family genes in maize

作　　者：藏金萍[2,3] 朱倩洁 邢超 曹宏哲 董金皋 张康 邢继红 ZANG Jin-ping;ZHU Qian-jie;XING Chao;CAO Hong-zhe;DONG Jin-gao;ZHANG Kang;XING Ji-hong(State Key Laboratory of North China Crop Improvement and Regulation,Baoding 071000,China;College of Life Sciences,Hebei Agricultural University,Baoding 071000,China;Hebei Key Laboratory of Plant Physiology and Molecular Pathology,Baoding 071000,China)

机构地区：[1]省部共建华北作物改良与调控国家重点实验室,保定071000 [2]河北农业大学生命科学学院,保定071000 [3]河北省植物生理与分子病理学重点实验室,保定071000

出　　处：《植物病理学报》2021年第3期340-350,共11页Acta Phytopathologica Sinica

基　　金：粮食丰产增效科技创新专项(2016YFD0300704);国家玉米产业技术体系(CARS-02-25);河北农业大学科研启动项目(ZD201721)。

摘　　要：SPXs(SPX-domain-containing proteins)家族基因在真核生物无机磷酸盐(Pi)的传感、信号转导和转运中发挥着重要作用,但玉米SPXs家族基因的相关研究未见报道。本研究利用生物信息学手段鉴定了15个玉米SPXs家族基因,通过系统进化和保守结构域分析将其分为SPX-EXS、SPX、SPX-Zinc finger和SPX-MFS 4个亚家族。通过表达谱数据分析,发现ZmSPXs家族基因具有明显的组织特异性表达。同时,我们还发现ZmSPXs家族基因在生物胁迫和非生物胁迫过程中表达模式也具有很大差异,说明这些基因可能在不同的生物过程中发挥重要作用。最后,利用荧光实时定量PCR技术,对玉米SPXs家族基因在磷胁迫处理下的表达规律进行分析,结果发现大部分基因在磷胁迫处理下呈现高水平表达。本研究鉴定了玉米SPXs家族基因,明确了该家族基因的系统进化关系、保守结构域、组织表达特性以及在生物和非生物胁迫条件下的表达规律,为阐明玉米SPXs家族基因的功能及其机制奠定了理论基础。SPX-domain-containing proteins(SPXs)play an important role in inorganic phosphate(Pi)sensing,signaling,and transport in eukaryotes.Related studies on SPXs of maize have not been reported.In this study,15 SPXs were obtained by searching the database of Pfam(http://pfam.sanger.ac.uk/)using bioinformatics methods,and were divided into SPX-EXS,SPX,SPX-Zinc finger,and SPX-MFS subfamilies by phylogenetic analysis and conserved domain analysis.The tissue expression specificity analysis of ZmSPXs found that different genes had significantly different expression levels in different developmental stages and different tissues,showing obvious tissue specificity.ZmSPXs family genes have specific high and low expression genes under heat,cold,salt,UV,and drought abiotic stress.The gene expression levels of ZmSPXs family genes in Fusarium verticillioides infection in different periods are obviously different.RT-qPCR was used to analyze the expression levels of ZmSPXs under phosphorus stress,and the results showed that most of the genes were highly expressed under phosphorus stress.This study clarified the number of genes,phylogenetic relationships,conserved domains,tissue expression characteristics and expression patterns under biological and abiotic stresses of SPXs in maize,which could provide basic data for clarifying the function and mechanism of SPXs.

关 键 词：玉米 SPXs 系统进化分析 基因表达 磷胁迫 

分 类 号：S435.131.4[农业科学—农业昆虫与害虫防治]