艾塞那肽对人卵巢癌OVCAR-3细胞凋亡侵袭和迁移的影响及机制  

Effect and mechanism of exenatide on apoptosis,invasion and migration of OVCAR-3 human ovarian cancer cell line

作　　者：王兰妮[1] 宁静 王婕 WANG Lan-ni;NING Jing;WANG Jie(Department Pathology,Baoji Maternal and Child Health Hospital,Baoji 721000,China;Department of Obstetrics and Gynecology,Xi’an Third Hospital,Xi’an 710001,China)

机构地区：[1]陕西省宝鸡市妇幼保健院病理科,宝鸡721000 [2]陕西省西安市第三医院妇产科,西安710001

出　　处：《中国肿瘤临床与康复》2021年第6期664-668,共5页Chinese Journal of Clinical Oncology and Rehabilitation

基　　金：陕西省自然科学基础研究计划项目(2016SY0327)。

摘　　要：目的探讨艾塞那肽对人卵巢癌OVCAR-3细胞凋亡、侵袭和迁移的影响及机制。方法选取人卵巢癌OVCAR-3细胞作为研究对象,并分为艾塞那肽组和对照组,艾塞那肽组给予对数生长期的人卵巢癌OVCAR-3细胞10-100μM的艾塞那肽处理,对照组不给予艾塞那肽处理。采用CCK-8法检测细胞活力,并计算其半数抑制浓度(IC50)。在确定的IC50浓度基础上,采用Transwell实验检测OVCAR-3细胞侵袭和迁移能力,流式细胞术检测细胞周期,实时定量聚合酶链式反应(PCR)法检测凋亡相关蛋白Bax和Bcl-2的表达情况,免疫印迹法检测核转录因子kappa-B(NF-κB)的表达及其乙酰化水平。结果 CCK-8试验结果显示,艾塞那肽对OVCAR-3细胞的半数抑制浓度(IC50)为40.08μM。与对照组相比,艾塞那肽可使人卵巢癌OVCAR-3细胞凋亡和细胞凋亡相关蛋白Bax的表达水平升高,细胞侵袭数、细胞迁移数、Bcl-2、NF-κB以及乙酰化NF-κB(acyl-NF-κB)的表达降低,差异均有统计学意义(均P<0.05)。结论艾塞那肽可抑制人卵巢癌OVCAR-3细胞的活力,促进其凋亡并抑制其侵袭和迁移能力,其作用机制可能与抑制NF-κB及其乙酰化表达有关。Objective To investigate the effect of exenatide on the apoptosis,invasion and migration of OVCAR-3 human ovarian cancer cells line. Methods The OVCAR-3 human ovarian cancer cells were selected and divided into an exenaptide group and a control group. For the exenaptide group,human ovarian cancer OVCAR-3 cells was given 10 to 100 μm exenatide during logarithmic growth phase,while the control group was not processed with exenatide. Cell viability was detected by CCK-8 kit. Half inhibit concentration( IC50) was calculated. Based on the determined IC50 concentration,the invasion and migration of OVCAR-3 cells were detected using Transwell test. Cell cycle was detected by flow cytometry. The expression of apoptosis-related proteins Bax and Bcl-2 was detected by real-time quantitative PCR. The expression and acetylation levels of NF-κB were detected by western blotting. Results CCK-8 test showed that the IC50 of exenatide for OVCAR-3 cells was 40. 08 μM. Compared with the control group,exenatide significantly increased the apoptosis of human ovarian cancer OVCAR-3 cells,the expression of Bax,the number of invasive cells,the number of cell migration,and the expression of Bcl-2,NF-κB and acetylated NF-κB( acyl NF-κB)( all P<0.05). Conclusion Exanaptide can inhibit the activity of OVCAR-3 human ovarian cancer cells,promote the apoptosis,and inhibit the invasion and migration. The mechanism of action may be related to the inhibition of NF-κB and its acetylation expression.

关 键 词：艾塞那肽 卵巢肿瘤 凋亡 侵袭 NF-ΚB 

分 类 号：R737.31[医药卫生—肿瘤]