Recognition of H3K9me1 by maize RNA-directed DNA methylation factor SHH2  

作　　者：Yuhua Wang Xuelin Zhou Jinyan Luo Suhui Lv Rui Liu Xuan Du Bei Jia Fengtong Yuan Heng Zhang Jiamu Du 

机构地区：[1]National Key Laboratory of Plant Molecular Genetics and Shanghai Center for Plant Stress Biology,Center for Excellence in Molecular Plant Sciences,The Chinese Academy of Sciences,Shanghai 201602,China [2]University of Chinese Academy of Sciences,Beijing 100049,China [3]School of Life Science,Key Laboratory of Molecular Design for Plant Cell Factory of Guangdong Higher Education Institutes,Institute of Plant and Food Science,School of Life Sciences,Southern University of Science and Technology,Shenzhen 518055,China

出　　处：《Journal of Integrative Plant Biology》2021年第6期1091-1096,共6页植物学报（英文版）

基　　金：supported by National Natural Science Foundation of China(31770782);the Ministry of Science and Technology of China(2016YFA0503200);the Shenzhen Science and Technology Program(JCYJ20200109110403829 and KQTD20190929173906742);Key Laboratory of Molecular Design for Plant Cell Factory of Guangdong Higher Education Institutes(2019KSYS006)to J.D.

摘　　要：RNA-directed DNA methylation(Rd DM) is a plant-specific de novo DNA methylation pathway,which has extensive cross-talk with histone modifications. Here, we report that the maize RdDM regulator SAWADEE HOMEODOMAIN HOMOLOG 2(SHH2) is an H3 K9 me1 reader. Our structural studies reveal that H3 K9 me1 recognition is achieved by recognition of the methyl group via a classic aromatic cage and hydrogen-bonding and salt-bridge interactions with the free protons of the mono-methyllysine. The di-and tri-methylation states disrupt the polar interactions, decreasing the binding affinity. Our study reveals a monomethyllysine recognition mechanism which potentially links RdDM to H3 K9 me1 in maize.

关 键 词：EPIGENETICS H3K9me1 MAIZE RNA-directed DNA methylation SAWADEE domain SHH2 

分 类 号：Q943[生物学—植物学]