节律基因Per1在咽喉部鳞癌中的表达及与临床病理和预后的相关研究  

Expression of Rhythm Gene Per1 in Pharynx/Larynx Squamous Cell Carcinoma and Its Correlation with Clinicopathology and Prognosis

作　　者：王苗 金风 李媛媛 吴伟莉 龙金华 罗秀玲 龚修云 陈潇潇 Wang Miao;Jin Feng;Li Yuanyuan;Wu Weili;Long Jinhua;Luo Xiuling;Gong Xiuyun;Chen Xiaoxiao(Department of Oncology,College of Clinical Medicine,Guizhou Medical University,Guiyang 550001,Guizhou,China;Department of Head and Neck Oncology y the Affiliated Cancer Hospital of Guizhou Medical University,Guiyang 550000,Guizhou,China)

机构地区：[1]贵州医科大学临床医学院肿瘤学教研室,贵阳550001 [2]贵州医科大学附属医院·贵州省肿瘤医院头颈肿瘤科,贵阳550000

出　　处：《肿瘤预防与治疗》2021年第6期531-537,共7页Journal of Cancer Control And Treatment

基　　金：贵州省教育厅创新群体重大研究项目(编号:黔教合KY字[2017]038)。

摘　　要：目的:探索节律基因Per1在咽喉部鳞癌中的表达及与临床病理和预后的相关性。方法:收集2015年10月至2016年9月贵州省肿瘤医院头颈肿瘤科咽喉部鳞癌患者的术后病理标本15例及13例咽喉部活检患者病理标本证实为非肿瘤的正常组织标本,采用RT-qPCR法检测两组标本中Per1 mRNA的表达水平;收集2013年1月至2017年3月60例经病理确诊为咽喉部鳞癌患者的存档蜡块及其中的20例癌旁组织蜡块,同时收集对应患者的临床病理资料,采用免疫组化法检测两组标本中Per1蛋白的表达情况,并分析Per1蛋白表达水平与患者临床病理发展及预后相关性。结果:Per1 mRNA在咽喉部鳞癌组织中的表达水平显著低于咽喉部正常组织中的表达水平(P<0.05),且局部晚期咽喉部鳞癌患者标本中的Per1 mRNA表达水平明显低于早期咽喉部鳞癌患者标本中的表达水平(P<0.05)。Per1在咽喉部鳞癌组织中的蛋白表达水平明显低于癌旁组织中的表达水平(P<0.05)。通过Kaplan-Meier统计学分析,Per1蛋白阳性表达组3年总生存率和无进展生存率明显高于阴性组,差异有统计学意义(P<0.05)。结论:节律基因Per1在咽喉部鳞癌组织中的表达显著下调,且其表达水平与咽喉部鳞癌患者的生存呈正相关,可能成为咽喉部鳞癌患者预后的重要标志物。Objective: To explore the expression of rhythm gene Per1 in pharynx/larynx squamous cell carcinoma and its correlation with clinicopathology and prognosis. Methods: From October 2015 to September 2016, 15 postoperative pathological specimens of pharynx/larynx squamous cell carcinoma, as well as 13 samples of normal tissues from pharynx/larynx confirmed by biopsies were collected from the department of head and neck oncology of the Affiliated Cancer Hospital of Guizhou Medical University. The expression levels of Per1 mRNA in two groups were detected by RT-qPCR. The archived wax blocks of 60 cases of pharynx/laryngeal squamous cell carcinoma and 20 cases of paracancerous tissues were collected from January 2013 to March 2017, and the clinicopathological data of those patients were collected. The expression levels of Per1 protein in two groups were detected by immunohistochemical method, and the correlation between the expression level of Per1 protein and the clinicopathological development and prognosis of the patients was analyzed. Results: The expression levels of Per1 mRNA in pharynx/larynx squamous cell carcinoma were significantly lower than those in normal pharynx/larynx tissues, and those in locally advanced larynx squamous cell carcinoma was significantly lower than those in early pharynx/larynx squamous cell carcinoma(P<0.05). The expression levels of Per1 protein in pharynx/larynx squamous cell carcinoma were significantly lower than those in paracancerous tissues. Kaplan-Meier analysis showed that both the 3-year overall survival and progression-free survival in the Per1+ group were significantly higher than those in the Per1-group(P<0.05). Conclusion: The expression of rhythm gene Per1 in pharynx/larynx squamous cell carcinoma is relatively low, and its expression level is positively correlated to the survival of patients. It may become an important marker for the prognosis of patients with pharynx/larynx squamous cell carcinoma.

关 键 词：头颈部鳞癌 生物钟基因 PER1 RT-QPCR 免疫组化 

分 类 号：R739.91[医药卫生—肿瘤]