circPTPRA与miR-1252在胃癌中的表达及其对细胞生物学行为的影响  

The expression of circPTPRA and miR-1252 in gastric cancer and their influence on cell biological behavior

作　　者：吉鸿[1] 索丹 赵耀[1] 李顺乐 徐心[1] 常帅 JI Hong;SUO Dan;ZHAO Yao;LI Shun-le;XU Xin;CHANG Shuai(General Surgery Department,the 2 nd Affiliated Hospital of Xi′an Jiaotong University,Xi′an 710004,Shaanxi;General Surgery Department,the 1 st Affiliated Hospital of Xi′an Jiaotong University,Xi′an 710061,Shaanxi)

机构地区：[1]西安交通大学第二附属医院普通外科,710004 [2]西安交通大学第一附属医院普通外科,710061

出　　处：《现代消化及介入诊疗》2021年第5期591-596,602,共7页Modern Interventional Diagnosis and Treatment in Gastroenterology

基　　金：陕西省自然科学基础研究计划(2019JQ-967)。

摘　　要：目的探讨circPTPRA与miR-1252在胃癌中的表达及其对胃癌细胞生物学行为的影响。方法取术中切除的胃癌组织与癌旁组织(距癌组织>5 cm处的组织)进行实验,随后采用qRT-PCR检测胃癌组织、癌旁组织中circPTPRA与miR-1252的表达情况。体外培养人胃癌细胞HGC-27,分别转染pcDNA、pcDNA-circPTPRA、si-NC、si-circPTPRA、anti-miR-NC、anti-miR-1252、共转染pcDNA-circPTPRA和miR-NC、共转染pcDNA-circPTPRA和miR-1252 mimics。CCK-8实验检测细胞增殖;流式细胞仪检测细胞周期;Transwell小室实验检测细胞迁移,Transwell Matrigel体外侵袭实验检测细胞侵袭;双荧光素酶报告基因实验检测circPTPRA与miR-1252的靶向关系;Western blot检测MMP-2、MMP-9蛋白表达量。结果与癌旁组织比较,胃癌组织中circPTPRA的表达量显著降低(P<0.05),miR-1252的表达量显著升高(P<0.05)。与转染pcDNA组比较,转染pcDNA-circPTPRA组细胞活力显著降低,G0/G1期细胞比例显著升高,S期细胞比例显著降低,细胞迁移数及侵袭数显著减少,MMP-2、MMP-9蛋白的表达水平显著降低,差异均有统计学意义(P<0.05)。circPTPRA能够靶向结合miR-1252;转染pcDNA-circPTPRA组miR-1252的相对表达量显著降低(P<0.05);转染si-circPTPRA组miR-1252的相对表达量显著升高(P<0.05)。与转染anti-miR-NC组比较,转染anti-miR-1252组细胞活力显著降低,G0/G1期细胞比例显著升高,S期细胞比例显著降低,细胞迁移及侵袭数显著减少,MMP-2、MMP-9蛋白表达水平显著降低,差异均有统计学意义(P<0.05)。与共转染pcDNA-circPTPRA和miR-NC组比较,共转染pcDNA-circPTPRA和miR-1252 mimics组细胞活力显著升高,G0/G1期细胞比例显著降低,S期细胞比例显著升高,迁移及侵袭细胞数显著增多,MMP-2、MMP-9蛋白表达水平显著升高,差异均有统计学意义(P<0.05)。结论circPTPRA过表达可通过负向调控miR-1252而抑制胃癌细胞增殖、迁移、侵袭及诱导细胞周期阻滞。Objective To explore the expression of circPTPRA and miR-1252 in gastric cancer and its influence on the biological behavior of gastric cancer cells.Methods The resected gastric cancer tissue and adjacent tissue(the tissue more than 5 cm away from the cancer tissue)were taken for experiment,then qRT-PCR was used to detect the expression of circPTPRA and miR-1252 in gastric cancer tissues and adjacent tissues.Human gastric cancer cell line HGC-27 was cultured in vitro and then transfected with pcDNA,pcDNA-circPTPRA,si-NC,si-circPTPRA,anti-miR-NC,anti-miR-1252,co-transfected with pcDNA-circPTPRA and miR-NC,and co-transfected with pcDNA-circPTPRA and miR-1252 mimics.CCK-8 assay was used to detect cell proliferation,flow cytometry was used to detect cell cycle,transwell chamber assay was used to detect cell migration,transwell Matrigel in vitro invasion experiment was used to detect cell invasion,double luciferase reporter gene assay was used to detect the targeting relationship between circPTPRA and miR-1252,Western blot was used to detect the protein expression of MMP-2 and MMP-9.Results Compared with adjacent tissues,the expression of circPTPRA in gastric cancer tissues was significantly decreased(P<0.05),while the expression of miR-1252 was significantly increased(P<0.05).Compared with the pcDNA transfected group,the cell viability of pcDNA-circPTPRA transfected group was decreased,the proportion of G0/G1-phase cells was increased,the proportion of S-phase cells was decreased,the numbers of cell migration and cell invasion were reduced,and the expression levels of MMP-2 and MMP-9 protein were decreased,and the differences were all statistically significant(P<0.05).circPTPRA could target miR-1252;the relative expression of miR-1252 in pcDNA-circPTPRA transfected group was significantly decreased(P<0.05),and that in si-circPTPRA transfected group was significantly increased(P<0.05).Compared with the anti-miR-NC transfected group,the cell viability of anti-miR-1252 transfected group was decreased,the proportion of G

关 键 词：circPTPRA miR-1252 胃癌 细胞增殖 迁移 侵袭 

分 类 号：R735.2[医药卫生—肿瘤] R573[医药卫生—临床医学]