Application of immunohistochemistry technique in hydrobiological studies  

作　　者：Hui Zhang Wen Wen Jizhou Yan 

机构地区：[1]Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources,Ministry of Education,Shanghai Ocean University,Shanghai 201306,China [2]Institute for Marine Biosystem and Neuroscience,Department of Biology,College of Fisheries and Life Sciences,Shanghai Ocean University,Shanghai 201306,China

出　　处：《Aquaculture and Fisheries》2017年第3期140-144,共5页渔业学报（英文）

基　　金：This work was supported in part by the International Cooperation Initiative Program of Shanghai Ocean University(A2302110002)to J.Yan;Shanghai Universities Firstclass Disciplines Project of Fisheries.

摘　　要：There is growing demand for biotechniques in oceanobiological and hydrobiological studies.Immunohistochemistry(IHC)combines anatomical,immunological and biochemical techniques to identify specific antigen-antibody interactions.Detecting the target antigen with labeled antibodies is a multi-step process that requires optimization at every level to maximize the signal detection.Here we use zebrafish samples and optimize each IHC step,particularly antigen retrieval,transgenic reporter double staining and background counterstaining.The resulting images with high signal intensity lead us to recommend the resulting protocols for experiments with fish.

关 键 词：IMMUNOHISTOCHEMISTRY PROTOCOL Transgenic fish HYDROBIOLOGY 

分 类 号：R73[医药卫生—肿瘤]