Isolation and characterization of a heat-resistant strain with high yield of Pyropia yezoensis Ueda(Bangiales,Rhodophyta)  

作　　者：Hongchang Ding Binglei Zhang Xinghong Yan 

机构地区：[1]College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 201306,PR China

出　　处：《Aquaculture and Fisheries》2016年第1期24-33,共10页渔业学报（英文）

基　　金：the National High Technology Research&Development Program of China(“863”Program)(Grant No.2006AA10A413);the National Natural Science Foundation of China(Grant No.31072208);the Shanghai Natural Science Foundation(Grant No.05RZ14110);Science and Technology Commission of Shanghai Municipality(Grant No.07XD14028);Shanghai Municipal Education Commission(Project No.J50701).

摘　　要：A heat-resistant Pyropia yezoensis strain YZ-4 was isolated from the regenerated blades of single somatic cells of the wild-type(WT)blades treated with 60Co-g-irradiation.When cultured at 23
C and 24
C,the survival and division rates of the YZ-4 conchospores were significantly higher than those of WT(P<0.01).After 16 days in culture the survival rates of YZ-4 conchospores were 71.2%and 60.8%at 23
C and 24
C,respectively,and the division rates of the surviving conchospores were 100%in both cases.Under the same culture conditions,the survival rates of WT conchospores were only 16.9%and 11.5%,and the division rate 99.8%and 81.8%respectively.The resistance of 50-day-aged F1 gametophytic blades of YZ-4 to the temperature rebound was also significantly higher than those of WT(P<0.01).After 30 days in culture at 18
C,23
C and 24
C,the mean blade length increased by 8.5,4.3 and 2.3 times in YZ-4 and was only 3,0.6 and 0.4 times in WT,respectively.The mean blade weight increased 191.9,85.4 and 66.2 times in YZ-4,but only 78.2,7.8 and 4.6 times in WT.On the 15th day WT blades began to decay,but no decay was observed in YZ-4 blades even after 45 days in culture at 23
C and 24
C.The results obtained indicate that YZ-4 has a higher resistance to higher temperature insults than WT and it might be of value for commercial production of P.yezoensis.

关 键 词：Pyropia yezoensis HEAT-RESISTANT Gametophytic blade Growth STRAIN Conchospore 

分 类 号：R73[医药卫生—肿瘤]