中老年2型糖尿病牙周炎患者龈沟液抗菌肽水平及其与牙周临床指标的相关性  

The level of antimicrobial peptides in gingival crevicular fluid and its correlation with periodontal clinical indexes in elderly patients with type 2 diabetic periodontitis

作　　者：张雅萌 张惠媛 阮世红[2] 陈晓春 甘雪琦 于海洋[1] ZHANG Yameng;ZHANG Huiyuan;RUAN Shi-hong;CHEN Xiaochun;GAN Xueqi;YU Haiyang(State Key Laboratory of Oral Diseases&National Clinical Re-search Center for Oral Diseases&West China Hospital of Stomatology,Sichuan University,Chengdu 610041,China;Shenzhen Center for Chronic Disease Control,Shenzhen 518000,China)

机构地区：[1]口腔疾病研究国家重点实验室国家口腔疾病临床研究中心四川大学华西口腔医院,四川成都610041 [2]深圳市慢性病防治中心,广东深圳518000

出　　处：《口腔疾病防治》2021年第11期746-751,共6页Journal of Prevention and Treatment for Stomatological Diseases

基　　金：十三五国家重点研发计划项目(2016YFC1102704)。

摘　　要：目的探究牙周炎合并2型糖尿病(type 2 diabetes mellitus,T2DM)患者龈沟液中抗菌肽人β防御素2(human betadefensin2,HBD2)和LL37的水平变化规律及其与牙周临床指标的相关性。方法选取于深圳市慢性病防治中心口腔科和内科就诊的中老年人(45至85岁)为研究对象,分为全身健康并牙周健康对照组22人、全身健康牙周炎组19人、T2DM牙周健康组15人、T2DM合并牙周炎组21人。采用佛罗里达牙周探针进行牙周检查,记录受试者牙周临床指标,包括探诊深度(probing depth,PD)、附着水平(attachment level,CAL)和探诊出血(probing bleeding,BOP)。采用酶联免疫吸附法检测龈沟液中抗菌肽HBD2、LL37浓度,比较组间抗菌肽HBD2、抗菌肽LL37及牙周临床指标间的差异,并进行相关性分析。结果T2DM合并牙周炎组的PD值大于全身健康牙周炎组(P<0.05);全身健康牙周炎组龈沟液中HBD2和抗菌肽LL37水平均高于全身健康并牙周健康对照组(P<0.05),全身健康牙周炎组龈沟液中HBD2水平高于T2DM合并牙周炎组(P<0.05)。全身健康牙周炎组龈沟液中抗菌肽HBD2、LL37与PD、CAL呈正相关(P<0.05),T2DM合并牙周炎组龈沟液中抗菌肽HBD2、LL37与PD、CAL相关性无统计学意义(P>0.05)。结论中老年T2DM牙周炎患者龈沟液抗菌肽HBD2、LL37水平较低,与牙周临床指标PD、CAL无显著相关性。Objective To investigate the changes and significance of human beta-defensin-2(HBD-2) and LL-37 in the gingival crevicular fluid of patients with periodontitis and type 2 diabetes mellitus(T2DM).Methods This study was conducted among 45-to 85-year-old patients in the Department of Stomatology and Internal Medicine of Shenzhen Center for Chronic Disease Control, including a healthy control group of 22 people, a systemically healthy control group of 19 people with periodontitis, a T2DM periodontal health group of 15 people, and a T2DM group of 21 people with periodontitis. The Florida periodontal probe was used for periodontal examination, and the clinical indexes, including probing depth(PD), clinical attachment level(CAL) and probing on bleeding(BOP), were recorded. The concentrations of HBD-2 and Ll-37 in gingival crevicular fluid were determined by ELISA. The differences in HBD-2, LL-37 and periodontal clinical indexes between the groups were compared, and correlation analysis was conducted.Results values in T2DM with the periodontitis group were higher than those of the systemically healthy controls with periodontitis group(P < 0.05);the levels of HBD-2 and LL-37 in gingival crevicular fluid in systemically healthy controls with periodontitis group were significantly higher than those in the healthy control group(P < 0.05), the level of HBD-2 in gingival crevicular fluid in systemically healthy controls with periodontitis group was significantly higher than that in T2DM with periodontitis group(P < 0.05);and the antimicrobial peptides HBD-2 and LL-37 in gingival crevicular fluid were significantly positively correlated with the PD and CAL in systemically healthy controls with periodontitis group(P < 0.05), and there was no significant correlation between the antimicrobial peptides HBD-2, LL-37 in gingival crevicular fluid and PD, CAL in T2DM with periodontitis group(P > 0.05).Conclusion The levels of antimicrobial peptides HBD-2 and LL-37 in gingival crevicular fluid of middle-aged and elderly patients with T2

关 键 词：中老年 2型糖尿病 牙周炎 龈沟液 抗菌肽 LL37 人Β防御素2 探诊深度 附着水平 探诊出血 

分 类 号：R78[医药卫生—口腔医学]