用NMR方法研究三尖杉酯碱诱导HL-60细胞的凋亡  

NMR STUDIES Of HARRINGTONINE INDUCED APOPTOSIS IN HL-60 CELLS

作　　者：吴昌琳[1] 钱旻[2] 黄永仁[1] 

机构地区：[1]华东师范大学分析测试中心 [2]华东师范大学生物系,上海200062

出　　处：《波谱学杂志》2002年第4期353-358,共6页Chinese Journal of Magnetic Resonance

基　　金：国家自然科学基金资助项目 (3 9870 649);上海市科委重点资助项目 (0 0JC1 40 49)

摘　　要：运用核磁共振波谱 (NMR)研究三尖杉酯碱 (HT)诱导人早幼粒白血病细胞 (HL 60 )凋亡中三磷酸腺苷 (ATP)、磷酸单脂 (PME)及脂类的代谢变化 .经HT作用 2h后 ,从31 PNMR中发现HL 60细胞中ATP、PME含量减少 ;而在1 HNMR中甲基峰有明显的增强 ,胆碱峰稍微减弱 .这些现象的研究将有利于增进对细胞凋亡发生机制的认识 .NMR spectroscopy methods were used to study the metabolic changes of ATP, PME and lipid during harringtonine (HT) induced apoptosis in HL-60 cell. After HL-60 cells were treated with HT for two hours, it was observed by 31P-NMR spectroscopy that the levels of ATP and PME decreased significantly and intracellular pH increased significantly. It was also found by 1H-NMR a significant increase in the signal intensity of methylene resonance and a slight decrease in choline peak in treated HL-60 cells. The present investigation will be helpful for understanding the mechanism of Harringtonine induced apoptosis in HL-60 cells.

关 键 词：NMR HL-60细胞 三尖杉酯碱 细胞凋亡 白血病 肿瘤细胞 治疗 核磁共振 药物诱导 

分 类 号：R733.7[医药卫生—肿瘤] R730.[医药卫生—临床医学]