温度、pH值和H_(2)O_(2)对减毒沙门氏菌VNP20009的生长及生物膜形成的影响  

Effects of temperature,pH,and H_(2)O_(2) on the growth and biofilm formation of attenuated Salmonella VNP20009

作　　者：李静 包斐斐 李家璜[1,2,3] 华子春 LI Jing;BAO Fei-fei;LI Jia-huang;HUA Zi-chun(State Key Laboratory of Pharmaceutical Biotechnology,School of Life Sciences,Nanjing University,Nanjing 210023,China;College of Biopharmaceuticals,China Pharmaceutical University,Nanjing 211198,China;High-Tech Research Institute of Nanjing University at Changzhou and Jiangsu Target Pharma Laboratories Inc.,Changzhou 213164,China)

机构地区：[1]南京大学生命科学学院,医药生物技术国家重点实验室,江苏南京210023 [2]中国药科大学生物药物学院,江苏南京211198 [3]常州南京大学高新技术研究院和江苏靶标生物医药研究所,江苏常州213164

出　　处：《药学学报》2021年第6期1606-1611,共6页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金资助项目(81630092,81573338);江苏省科技厅项目(BK20192005,BK20171202).

摘　　要：减毒沙门氏菌VNP20009具有较好的在肿瘤中定殖、复制并发挥一定抗肿瘤作用的特性,可作为抗癌药物载体或与其他疗法(如化疗)联用,具有较好应用前景。本文对VNP20009的生物学性质进行研究,检测了VNP20009在不同温度、pH值及H_(2)O_(2)作用下的生长曲线及细菌生物膜的形成。结果表明,VNP20009在42℃、弱酸性环境pH 6.5以及H_(2)O_(2)(1 mmol·L^(-1))条件下可正常生长,弱酸性环境有益于VNP20009生物膜的形成。本实验结果对深入研究减毒沙门氏菌的生存机制和应用提供了基础。Attenuated Salmonella VNP20009 specifically colonizes and proliferates within tumor tissues and inhibits tumor growth.It has been used as drug delivery vehicle or in combination with other therapies(such as chemotherapy),which shows a good application potential in tumor therapy.In this paper,study was conducted to determine the physiological changes of growth curve and formation of bacterial biofilm of VNP20009 under various environmental stresses,such as temperature,pH,and H_(2)O_(2).The results showed that VNP20009 could grow normally under the conditions of 42℃,pH 6.5,and 1 mmol·L^(-1) H_(2)O_(2).Furthermore,the weak acid environment was beneficial to the biofilm formation of VNP20009.This study provides a basis for in-depth study of the survival mechanism and application of attenuated Salmonella.

关 键 词：沙门氏菌 VNP20009 生长 生物膜 微生物学现象 

分 类 号：R966[医药卫生—药理学]