双清平化方对代谢综合征大鼠血压、胰岛素抵抗、RAS系统及血管内皮功能的影响  

Influence of Shuang Qing Ping Hua Decoction on blood pressure,insulin resistance,RAS system and vascular endothelial function in rats with metabolic syndrome

作　　者：王瑶瑶 喇孝瑾 张大伟 张敏 陈瑞军 付文浩 李继安 WANG Yaoyao;LA Xiaojin;ZHANG Dawei;ZHANG Min;CHEN Ruijun;FU Wenhao;LI Ji’an(School of Traditional Chinese Medicine,North China University of Science and Technology,Tangshan 063000,Hebei,China;School of Stomatology,North China University of Science and Technology,Tangshan 063000,Hebei,China)

机构地区：[1]华北理工大学中医学院,河北唐山063000 [2]华北理工大学口腔医学院,河北唐山063000

出　　处：《现代中西医结合杂志》2021年第20期2172-2178,共7页Modern Journal of Integrated Traditional Chinese and Western Medicine

基　　金：国家科技部科技援助项目(KY201904005);河北省科技厅国际合作项目(14397705D);河北省农林科学院财政项目(F18R18001);唐山市科学技术研究与发展计划项目(19130205C)。

摘　　要：目的观察双清平化方对代谢综合征大鼠血压、胰岛素抵抗、RAS系统及血管内皮功能的影响,探讨该方药降压的分子机制。方法取60只雄性SD大鼠,随机选6只作为正常组,剩余大鼠建立代谢综合征模型。将造模成功的30只代谢综合征大鼠随机分为模型组、缬沙坦组及双清平化方高、中、低剂量组各6只。缬沙坦组及双清平化方高、中、低剂量组分别予含缬沙坦及高、中、低剂量双清平化方的饲料喂养8周。测量各组大鼠实验开始(0周)及干预4周、8周的收缩压(SBP)、舒张压(DBP);干预8周后处死各组大鼠,检测血清空腹血糖(FPG)、胰岛素(FINS)、内皮素-1(ET-1)、一氧化氮(NO)、血管紧张素Ⅱ(AngⅡ)水平,计算稳态胰岛素抵抗指数(HOMA-IR);HE及Masson染色观察主动脉的病理变化;Weston blot检测肾脏组织中血管紧张素转换酶(ACE)、血管紧张素Ⅱ受体1型(AT1R)、血管紧张素Ⅱ受体2型(AT2R)蛋白表达水平。结果与模型组比较,双清平化方高剂量组SBP、DBP、FPG、FINS、HOMA-IR、ET-1、AngⅡ均明显降低(P均<0.05),NO明显升高(P<0.05)。HE染色和Masson染色显示,各药物组血管内膜与中膜的病理损伤和胸主动脉中胶原沉积均较模型组明显改善,以双清平化方高剂量组及缬沙坦组改善尤为显著。与模型组比较,各药物组肾脏组织中ACE、AT1R蛋白表达水平均显著降低(P均<0.05),AT2R蛋白表达水平均显著增加(P均<0.05),其中双清平化方高剂量组各蛋白表达水平与缬沙坦组比较差异均无统计学意义(P均>0.05)。结论双清平化方可降低代谢综合征大鼠的血压、血糖,改善胰岛素抵抗及血管内皮功能,降压机制推测与降低AngⅡ,下调肾脏ACE、AT1R蛋白表达,上调肾脏AT2R蛋白表达,抑制RAS的激活有关。Objective It is to observe the effect of Shuang Qing Ping Hua Decoction(SQPHD)on blood pressure,insulin resistance,RAS system and vascular endothelial function in rats with metabolic syndrome,and to explore the molecular mechanism of this prescription for reducing blood pressure.Methods Sixty male SD rats were selected,in which 6 ones were randomly selected as the normal group,and the metabolic syndrome models were established in the remaining rats.The 30 successful modeled rats with metabolic syndrome were randomly divided into model group,valsartan group and high,medium and low dose groups of SQPHD,6 rats in each group.The valsartan group and the high,medium and low dose groups of SQPHD were respectively fed with the feed containing valsartan and high,medium and low doses of SQPHD for 8 weeks.The systolic blood pressure(SBP)and diastolic blood pressure(DBP)of the rats in each group were detected at the beginning of the experiment(0 week)and after intervention for 4 weeks and 8 weeks;after 8 weeks of intervention,the rats in each group were sacrificed,the levels of serum fasting plasma blood glucose(FPG),insulin(FINS),endothelin-1(ET-1),nitric oxide(NO),angiotensinⅡ(AngⅡ)were determined,and steady-state insulin resistance index(HOMA-IR)was calculated;the aortic pathological changes were observed by HE and Masson staining;the protein expression levels of angiotensin converting enzyme(ACE),angiotensinⅡreceptor type 1(AT1R),and angiotensinⅡreceptor type 2(AT2R)in kidney tissue were detected by Weston blot was.Results Compared with the model group,SBP,DBP,FPG,FINS,HOMA-IR,ET-1,and AngⅡwere significantly reduced(all P<0.05),and NO was significantly increased in the high-dose SQPHD group(P<0.05).HE staining and Masson staining showed that the pathological damage of the vascular intima and media and the collagen deposition in the thoracic aorta in each treatment group were significantly improved compared with the model group.The improvement was particularly significant in the high-dose SQPHD group and the val

关 键 词：双清平化方 代谢综合征 血压 胰岛素抵抗 血管紧张素转换酶 血管紧张素Ⅱ 血管内皮功能 

分 类 号：R-332[医药卫生]