芪山配方颗粒治疗肥胖湿热蕴脾证及其对肠道菌群-免疫炎症途径的影响  

Effect of Qishan Formula Granule on Obesity Accumulation of Dampness and Heat in the Spleen Syndrome and Intestinal Flora-Immune Inflammatory Pathway

作　　者：魏燕[1] 金剑虹[1] 王雁秋[1] WEI Yan;JIN Jianhong;WANG Yanqiu(Hangzhou Hospital of Zhejiang Chinese Medical University,Hangzhou(310007),China)

机构地区：[1]浙江中医药大学附属杭州市中医院,杭州310007

出　　处：《浙江中医药大学学报》2021年第6期588-595,共8页Journal of Zhejiang Chinese Medical University

基　　金：浙江省中医药科技计划项目(2019ZA083)。

摘　　要：[目的]探讨自拟健脾清胃中药芪山配方颗粒干预单纯性肥胖湿热蕴脾证的疗效以及对肠道菌群-免疫炎症途径的影响。[方法]将2019年1月1日至12月31日浙江中医药大学附属杭州市中医院收治的80例单纯性肥胖湿热蕴脾证患者随机分组为中药组和对照组,中药组患者给予芪山配方颗粒治疗12周对照组患者给予安慰剂治疗12周。检测两组患者疗效治疗前后生化指标、临床症状、肠道菌群数量及组成、血清辅助性T细胞17(Thelper cell 17,Th17)/调节性T细胞(regulatory T cell,Treg)比例和炎症因子水平。根据Th17/Treg比例高低,将中药组患者分为高水平组和低水平组进一步分析两组的肠道菌群差异。[结果]治疗后中药组总有效率显著高于对照组(P<0.01)。与治疗前比较治疗后中药组患者生化指标和临床症状显著好转(P<0.05)患者肠道部分益生菌数量显著增加(P<0.05),白介素-17(interleukin-17,IL-17)、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)水平、Th17/Treg比例均显著降低(P<0.05)。与对照组比较治疗后中药组患者生化指标和临床症状显著好转(P<0.05)患者肠道部分益生菌数量显著增加(P<0.05),IL-17、TNF-α水平、Th17/Treg比例均显著降低(P<0.05)。Th17/Treg低水平组患者肠道益生菌总量显著高于Th17/Treg高水平组(P<0.05)。[结论]芪山配方颗粒能够通过肠道菌群-免疫炎症途径,减轻单纯性肥胖湿热蕴卑证患者临床症状,改善生化指标提高疗效,值得临床推广使用。[Objective]To investigate the effects of Qishan Formula Granule on obesity accumulation of dampness and heat in the spleen syndrome and intestinal flora-immune inflammatory pathway.[Methods]From January 1 to December 31,2019,eighty patients with simple obesity in Hangzhou Hospital of Zhejiang Chinese Medical University were randomly divided into two groups:Chinese medicine group and control group.Patients of Chinese medicine group were treated with Qishan Formula Granule,and patients of control group were treated with placebo for 12 weeks.The therapeutic effect,biochemical indexes,clinical symptoms,the number and composition of intestinal flora,the proportion of T helper cell 17(Th17)/regulatory T cell(Treg)in serum and the levels of inflammatory factors were measured before and after treatment.According to the proportion of Th17/Treg,the patients in Chinese medicine group were divided into high-level group and low-level group,and the differences of intestinal flora between the two groups were further analyzed.[Results]After treatment,the total effective rate of Chinese medicine group was significantly higher than that in control group(P<0.01).Compared with before treatment,the biochemical indexes and clinical symptoms of Chinese medicine group improved significantly after treatment(P<0.05),the quantity of partial probiotics of Chinese medicine group was increased(P<0.05),the levels of interleukin-17(IL-17),tumor necrosis factor-α(TNF-α)and the proportion of Th17/Treg of Chinese medicine group were significantly lower(P<0.05).Compared with control group,the biochemical indexes and clinical symptoms of Chinese medicine group improved significantly after treatment(P<0.05),the quantity of partial probiotics in Chinese medicine group was increased(P<0.05),and the levels of IL-17,TNF-α and the proportion of Th17/Treg of Chinese medicine group were significantly lower(P<0.05).The total amount of intestinal probiotics in patients with low Th17/Treg level was significantly higher than that in patients with high Th17/T

关 键 词：单纯性肥胖 湿热蕴脾证 胃强脾弱 芪山配方颗粒 肠道菌群 TH17/TREG IL-17 TNF-α 

分 类 号：R574[医药卫生—消化系统]