牦牛蹄筋胶原蛋白提取工艺优化  

Optimization of Extraction Process of Collagen from Yak Tendon

作　　者：孙培利 谈花 王晓燕 曹效海[1] 王树林[1,2] SUN Pei-li;TAN Hua;WANG Xiao-yan;CAO Xiao-hai;WANG Shu-lin(College of Agriculture and Animal Husbandry,Qinghai University,Xining 810016,Qinghai,China;State Key Laboratory of Plateau Ecology and Agriculture,Qinghai University,Xining 810016,Qinghai,China)

机构地区：[1]青海大学农牧学院,青海西宁810016 [2]青海大学省部共建三江源生态与高原农牧业国家重点实验室,青海西宁810016

出　　处：《食品研究与开发》2021年第13期119-125,共7页Food Research and Development

摘　　要：为高效制备牦牛蹄筋胶原蛋白,以牦牛蹄筋为原料,采用热水法和酶解法提取牦牛蹄筋胶原蛋白,选择合适的提取方法,通过单因素试验分析料液比、温度、时间、加酶量对牦牛蹄筋胶原蛋白得率、提取率及纯度的影响,以正交试验优化酶解条件。结果表明:酶解法提取牦牛蹄筋胶原蛋白效果较好,最佳提取条件为料液比1∶12(g/mL)、加酶量2.5%、温度43℃、时间11 h,胶原蛋白的得率、提取率及纯度分别为14.91%,69.94%和89.44%。In order to efficiently extract the collagen from yak tendon,the yak tendon was used as raw material to extract the collagen by hot water and enzymolysis method,and the best extraction method was selected.On the basis of the optimal extraction method,the effects of different ratios of solid-to-liquid,temperature,time and enzyme dosage on the yield,extraction rate and purity of yak tendon collagen were studied by single factor ex-periment.The extraction conditions were optimized by orthogonal experiment.The results showed that enzymol-ysis method had better effect on yak tendon collagen extraction.The optimal extractionconditionswere deter-mineas follows:solid-to-liquid ratio 1∶12(g/mL),and enzyme dosage 2.5%,and extraction at 43℃for 11 h.Under these conditions,the yield,extraction rate and purity of collagen were 14.91%,69.94%and 89.44%,re-spectively.

关 键 词：牦牛蹄筋 胶原蛋白 提取工艺 酶解法 正交试验 

分 类 号：TS251.92[轻工技术与工程—农产品加工及贮藏工程]