抗生素与重金属对蛋白核小球藻时间依赖协同作用的动态定量表征  

Dynamic and quantitative characterization of time-dependent synergism between antibiotics and heavy metals on Chlorella pyrenoidosa

作　　者：宋崇崇 陶梦婷 张瑾 骆纵纵 周娜娜 SONG Chongchong;TAO Mengting;ZHANG Jin;LUO Zongzong;ZHOU Nana(Key Laboratory of Water Pollution Control and Wastewater Resource of Anhui Province,College of Environment and Energy Engineering,Anhui Jianzhu University,Hefei,230601,China)

机构地区：[1]安徽建筑大学环境与能源工程学院,安徽省水污染控制与废水资源化重点实验室,合肥230601

出　　处：《环境化学》2021年第6期1691-1704,共14页Environmental Chemistry

基　　金：国家自然科学基金(21677001);安徽省省级质量工程项目(2020jyxm0355);安徽建筑大学校级质量工程(2020xgk02,2020jy75)资助.

摘　　要：以硫酸阿米卡星(Amikacin sulfate,AMI)、五水合氯化镉(CdCl_(2)·2.5H_(2)O,Cd)、四水合氯化锰(MnCl_(2)·4H_(2)O,Mn)和七水合硫酸锌(ZnSO_(4)·7H_(2)O,Zn)为研究对象,以蛋白核小球藻(Chlorella pyrenoidosa,C.pyrenoidosa)为指示生物,采用均匀设计射线法设计3组二元混合体系(AMI-Cd、AMI-Mn和AMI-Zn),并应用时间毒性微板分析法测定其对C.pyrenoidosa的毒性数据,以浓度加和模型(Concentration addition,CA)和独立作用模型(Independent action,IA)为标准加和模型进行毒性相互作用分析,并运用效应残差比(Effect residual ratio,ERR)进行定量动态表征毒性相互作用.结果表明,3组二元混合体系共十五条射线的混合毒性呈明显的时间依赖性和浓度依赖性;依据ERR,CA和IA对三组二元混合体系的毒性评估结果基本一致,但3个混合物体系的毒性相互作用具有不同的时间变化规律,即随暴露时间延长,AMI-Cd中五条射线的中浓度区由协同作用逐渐变为加和作用,AMI-Zn中5条射线在高浓度区的协同作用逐渐变为加和作用,而中低浓度区的协同作用在加强;AMI-Mn的毒性相互作用随时间变化不是很明显;与CA和IA相比,ERR定量地表征了混合物体系的毒性相互作用强度,这为客观和准确地评估污染物的环境风险提供了方法和数据参考.Amikacin sulfate(AMI),cadmium chloride pentahydrate(CdCl_(2)·2.5H_(2)O,Cd),manganese chloride tetrahydrate(MnCl_(2)·4H_(2)O,Mn)and zinc sulfate heptahydrate(ZnSO_(4)·7H_(2)O,Zn)were selected as the research objects to investigate their combined toxicity towards a freshwater algae Chlorella pyrenoidosa(C.pyrenoidosa)by the time-dependent toxicity microplate analysis.Three binary mixture systems(AMI-Cd,AMI-Mn and AMI-Zn)were designed by uniform design ray method.Concentration addition(CA)and independent action(IA)models were used to analyze toxicity interaction,and the effect residual ratio(ERR)was used to dynamically and quantitatively characterize toxic interaction.The results show that the combined toxicity of 15 rays in three binary systems is of obvious time-dependency and concentration-dependency.The toxicity interaction analyzed by CA and IA for the three binary mixture systems are similar,but different mixture systems have different time regularities.With the prolonging of exposure time,the medium concentration region of the five rays in AMI-Cd gradually changes from synergism to additive effect.The high concentration region of the five rays in AMI-Zn gradually changes from synergism to additive effect,and synergism is strengthening in the middle and low concentration regions.The changing of toxicity interaction within AMI-Mn mixture system with time is not obvious.In addition,compared with CA and IA,ERR quantitatively characterized the toxic interaction intensity of the mixture system,which provides a method and data reference for the objective and accurate assessment of the environmental risk of pollutants.

关 键 词：混合毒性 蛋白核小球藻 浓度加和模型 独立作用模型 效应残差比 

分 类 号：X171.5[环境科学与工程—环境科学]