A Triterpenoid Inhibited Hormone-Induced Adipocyte Differentiation and Alleviated Dexamethasone-Induced Insulin Resistance in 3T3-L1 adipocytes  

作　　者：Ji-Huan Qin Jun-Zeng Ma Xing-Wei Yang Ying-Jie Hu Juan Zhou Lin-Chun Fu Ru-Hua Tian Shan Liu Gang Xu Xiao-Ling Shen 

机构地区：[1]Laboratory of Chinese Herbal Drug Discovery,Tropical Medicine Institute,Guangzhou University of Chinese Medicine,Guangzhou 510405,People’s Republic of China [2]State Key Laboratory of Phytochemistry and Plant Resources in West China,Kunming Institute of Botany,Chinese Academy of Sciences,Kunming 650201,People’s Republic of China

出　　处：《Natural Products and Bioprospecting》2015年第3期159-166,共8页应用天然产物（英文）

基　　金：China National Major Projects of Science&Technology(2014ZX10005002;2009ZX09103-436);the Young Academic Leader Raising Foundation of Yunnan Province(No.2009CI073);the foundation from Chinese Academy of Sciences to Gang Xu,and the Program for Research Team in South China Chinese Medicine Collaborative Innovation Center of Guangdong,China(A1-AFD01514A07).

摘　　要：6a-Hydroxylup-20(29)-en-3-on-28-oic acid(1),a natural triterpenoid,was found to possess the ability in a dose-dependent manner inhibiting hormone-induced adipocyte differentiation in 3T3-L1 preadipocytes,and restoring glucose consuming ability in dexamethasone(DXM)-induced insulin resistant 3T3-L1 adipocytes.Compound 1 was also found to ameliorate DXM-induced adipocyte dysfunction in lipolysis and adipokine secretion.Mechanistic studies revealed that 1 inhibited adipocyte differentiation in 3T3-L1 preadipocytes via down-regulating hormone-stimulated gene transcription of peroxisome proliferator-activated receptor c and CCAAT-enhancer-binding protein alpha which are key factors in lipogenesis,and restored DXM-impaired glucose consuming ability in differentiated 3T3-L1 adipocytes via repairing insulin signaling pathway and activating down-stream signaling transduction by phosphorylation of signaling molecules PI3K/p85,Akt2 and AS160,thus leading to increased translocation of glucose transporter type 4 and transportation of glucose.

关 键 词：6a-Hydroxylup-20(29)-en-3-on-28-oic acid 3T3-L1 Adipocyte differentiation DEXAMETHASONE induced insulin resistance Adipocyte dysfunction PI3K/Akt2 signaling 

分 类 号：O62[理学—有机化学]