OCTA与FFA在小鼠脉络膜新生血管模型中应用的比较研究  

Comparison of consistency between optical coherence tomography angiography and fundus fluorescein angiography in a mouse model of laser-induced choroidal neovascularization model

作　　者：张万虎 刘轩[2] 李晶明[2] 谢安明[2] 康前雁[2] 陈丽[2] 齐赟 胡霄霏 ZHANG Wanhu;LIU Xuan;LI Jingming;XIE Anming;KANG Qianyan;CHEN Li;QI Yun;HU Xiaofei(East District,the First Affiliated Hospital of Xi’an Jiaotong University,Xi’an 710000,Shaanxi Province,China;Department of Ophthalmology,the First Affiliated Hospital of Xi’an Jiaotong University,Xi’an 710061,Shaanxi Province,China)

机构地区：[1]西安交通大学第一附属医院东院,陕西省西安市710000 [2]西安交通大学第一附属医院眼科,陕西省西安市710061

出　　处：《眼科新进展》2021年第7期628-632,共5页Recent Advances in Ophthalmology

基　　金：国家自然科学基金项目(编号81800824);陕西省自然科学基础研究计划项目(编号2020JM-400)。

摘　　要：目的以脉络膜铺片为金标准,探讨小鼠脉络膜新生血管(CNV)模型中荧光素眼底血管造影(FFA)与光学相干断层扫描血管成像(OCTA)描述小鼠CNV形态准确性的差异。方法应用激光诱导制作小鼠CNV模型,在激光造模后第7、14天分别利用FFA、OCTA进行小鼠眼底扫描,并与小鼠脉络膜铺片结果进行对比,寻找与脉络膜铺片一致性最高的检查技术。结果CNV模型小鼠眼底经测量的FFA、眼底激光成像、OCTA与脉络膜铺片中激光斑的面积,通过ICC一致性分析发现以脉络膜铺片作为金标准,与它一致性最显著的为FFA,眼底激光成像次之,缺乏一致性的为OCTA。以此得出结论对于小鼠CNV模型眼底CNV的形态,FFA检查与脉络膜铺片的一致性较好;OCTA与脉络膜铺片的一致性弱于FFA。结论OCTA、FFA以及多重眼底照相都可以描述小鼠CNV的形态,但FFA仍是描述CNV形态较为准确的工具。Objective To compare the consistency of fundus fluorescein angiography(FFA)and optical coherent tomography angiography(OCTA)for detecting lesion area with whole mount in vitro staining with isolectin-IB4 as gold standard in a mouse model of laser-induced choroidal neovascularization(CNV).Methods Adult mice were subjected to laser to induce a model of CNV.Fundus was scanned and CNV areas were measured by FFA and OCTA on day 7 and 14.CNV areas were measured in all different imaging modalities after laser and were compared to choroidal-RPE whole mounts to find out a better consistency test technology.Results After the calculation of the choroidal neovascularization area measurements in FFA imagine,multicolor imagine,OCTA and flat-mount,ICC correlation analysis showed there was a high correlation between FFA and flat-mount staining,while there was a poor consistency between OCTA and flat-mount staining.Therefore,it was concluded that for the CNV area in laser-induced CNV,FFA showed a better consistency with flat-mount staining than that between OCTA and flat-mount staining.Conclusion It is found that FFA and OCTA can be used to evaluate the CNV area in laser-induced mice.However,FFA has a better consistency with flat-mount staining than that between OCTA and flat-mount staining.

关 键 词：荧光素眼底血管造影 光学相干断层扫描血管成像 脉络膜新生血管 小鼠模型 

分 类 号：R773.4[医药卫生—眼科]